FORMATION OF PILIN IN PSEUDOMONAS-AERUGINOSA REQUIRES THE ALTERNATIVE SIGMA-FACTOR (RPON) OF RNA-POLYMERASE
FORMATION OF PILIN IN PSEUDOMONAS-AERUGINOSA REQUIRES THE ALTERNATIVE SIGMA-FACTOR (RPON) OF RNA-POLYMERASE
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DOI：
10.1073/pnas.86.6.1954
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发表时间：
1989-03-01
影响因子：
11.1
通讯作者：
LORY, S
中科院分区：
文献类型：
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作者：
ISHIMOTO, KS;LORY, S
铜绿假单胞菌pilin基因的启动子区域与肠道细菌的氮调控启动子高度相似。这些启动子被另一个。sigma识别。RNA聚合酶的因子，称为RpoN （NtrA或GlnF）。这一观察结果表明，铜绿假单胞菌毛蛋白基因可能是由含rpo的RNA聚合酶转录的。因此，我们将铜绿假单胞菌的RpoN基因克隆到大肠杆菌中（在大肠杆菌中形成功能性产物），并利用克隆的基因构建铜绿假单胞菌的突变体，该突变体的RpoN基因被插入失活。该突变体未能合成pilin，表明RpoN .sigma。该因子是匹林基因转录所必需的。
The promoter region of the Pseudomonas aeruginosa pilin gene has a high degree of similarity to the nitrogen-regulated promoters of enteric bacteria. These promoters are reconized by the alternative .sigma. factor of RNA polymerase, termed RpoN (NtrA or GlnF). This observation suggested that the P. aeruginosa pilin gene may be transcribed by the RpoN-containing RNA polymerase. We therefore, cloned the RpoN gene from P. aeruginosa into Escherichia coli (where it formed a functional product) and used that cloned gene to construct a mutant of P. aeruginosa that was insertinally inactivated in its RpoN gene. This mutant failed to synthesize pilin, indicating that the RpoN .sigma. factor is required for transcription of the pilin gene.