Quick Regulation of mRNA Functions by a Few Seconds of Photoirradiation,
Quick Regulation of mRNA Functions by a Few Seconds of Photoirradiation,
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通过几秒钟的光照射快速调节 mRNA 功能，
DOI：
10.1039/c2ob25883h
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发表时间：
2012
影响因子：
3.2
通讯作者：
Atsuo Shigeno
中科院分区：
文献类型：
--
作者：
Kenzo Fujimoto;Hiroki Yoshinaga;Yasumasa Yoshio and Takashi Sakamoto;Atsuo Shigeno
3-氰基乙烯基咔唑核苷，有效地光交联的嘧啶碱基互补RNA链，被纳入反义寡核苷酸，我们评估的光反应性和序列选择性突变的K-ras寡核苷酸，以及K-ras mRNA的功能的调节。我们证明，K-ras mRNA的逆转录和翻译活性被快速抑制的几秒钟的光照射与光响应的反义ODN的添加。
3-Cyanovinylcarbazole nucleoside, which effectively photocrosslinks to the pyrimidine base in complementary RNA strands, was incorporated into antisense oligonucleotides, and we evaluated the photoreactivity and the sequence selectivity to mutated K-ras oligoRNAs, as well as the regulation of the function of K-ras mRNA. We demonstrated that the reverse transcription and the translation activity of K-ras mRNA were quickly suppressed by a few seconds of photoirradiation with the addition of the photoresponsive antisense ODN.