Flow cytometric discrimination of various phycobilin-containing phytoplankton groups in a hypertrophic reservoir
Flow cytometric discrimination of various phycobilin-containing phytoplankton groups in a hypertrophic reservoir
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DOI：
10.1002/cyto.10104
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发表时间：
2002-05-01
期刊：
影响因子：
--
通讯作者：
Wilhelm, C
中科院分区：
文献类型：
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作者：
Becker, A;Meister, A;Wilhelm, C
背景：浮游植物结构的知识在水质控制中是重要信息。特别是湖泊修复和卫生措施必须在生物层面进行评估，以评估生物效应并评估潜在有毒蓝藻水华的风险。我们在一个正在修复的富营养化水库中使用并对比测试了三种独立的浮游植物分析方法。
方法：通过高效液相色谱色素分析、显微镜细胞计数以及流式细胞术（FCM）光散射和荧光分析对9种单藻培养物和室外样本进行检测，以测量主要藻类群对叶绿素a和生物量的百分比贡献。FCM仪器设置和鉴定标准是利用514nm的单一激发波长来区分代表主要藻类群的9种藻类而制定的。在585、620、650和680nm处检测荧光。
结果：结果表明，FCM是唯一一种能在叶绿素a和生物量基础上确定浮游植物组成变化的方法。
结论：这三种方法各有特定的优缺点，应根据实验问题进行选择。FCM分选允许将这三种方法结合起来，并提供了进一步的新视角。（C）2002威利 - 利斯公司
Background: Knowledge of phytoplankton structure is important information in water quality control. Lake restoration and sanitation measures in particular must be evaluated on the organismic level to valuate biological effects and assess the risk of potentially toxic Cyanobacteria blooms. We used and comparatively tested three independent methods for phytoplankton analysis in a hypertrophic reservoir under restoration.Methods: Nine unialgal cultures and outdoor samples were examined by high-performance liquid chromatography pigment analysis, microscopical cell counting, and flow cytometric (FCM) light scatter and fluorescence analysis to measure the percentage contribution of the major algal groups to chlorophyll a and biovolume, The FCM instrument settings and identification criteria were developed using a single excitation wavelength at 514 nm to differentiate nine algal species representing the major groups of algae. Fluorescence was detected at 585, 620, 650, and 680 nm.Results: The results show that FCM is the only method for determining changes in the phytoplankton composition on both a chlorophyll a and biovolume basis.Conclusions: Each of the three methods has specific advantages and disadvantages, and should be chosen depending on the experimental problem. FCM sorting allows the combination of all three and offers further new perspectives. (C) 2002 Wiley-Liss, Inc.