Metabolomic analysis of normal and sickle cell erythrocytes
Metabolomic analysis of normal and sickle cell erythrocytes
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DOI：
10.1016/j.tracli.2010.06.011
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发表时间：
2010-09-01
影响因子：
1.7
通讯作者：
Romeo, P. -H.
中科院分区：
文献类型：
--
作者：
Darghouth, D.;Koehl, B.;Romeo, P. -H.
我们使用一种简单且高度繁殖的红细胞代谢物提取方法，结合基于液相色谱-质谱的代谢物分析方法来测定正常红细胞和镰状红细胞的代谢组。镰状红细胞和正常红细胞的代谢组在糖酵解方面显示出主要差异。谷胱甘肽，抗坏血酸代谢和与膜更新相关的代谢物中红细胞内部分发生的尿素循环和一氧化氮代谢产生的代谢物的数量在正常红细胞和镰状细胞红细胞之间是不同的。这些结果表明，红细胞疾病的代谢分析现在可以确定，并可能指出新的生物标志物，可用于镰状细胞患者的随访(C)
Metabolic signatures of specialized circulating hematopoietic cells in physiological or human hematological diseases start to be described We use a simple and highly reproductive extraction method of erythrocytes metabolites coupled with a liquid chromatography-mass spectrometry based metabolites profiling method to determine metabolomes of normal and sickle cell erythrocytes Sickle cell erythrocytes and normal erythrocytes metabolomes display major differences in glycolysis. in glutathione, in ascorbate metabolisms and in metabolites associated to membranes turnover In addition, the amounts of metabolites derived from urea cycle and NO metabolism that partly take place within erythrocyte were different between normal and sickle cell erythrocytes These results show that metabolic profiling of red blood cell diseases can now be determined and might indicate new biomarkers that can be used for the follow-up of sickle cell patients (C) 2010 Published by Elsevier Masson SAS