TRIM31 interacts with p52Shc and inhibits Src-induced anchorage-independent growth
TRIM31 interacts with p52Shc and inhibits Src-induced anchorage-independent growth
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DOI：
10.1016/j.bbrc.2009.08.028
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发表时间：
2009-10-16
影响因子：
3.1
通讯作者：
Hatakeyama, Shigetsugu
中科院分区：
文献类型：
--
作者：
Watanabe, Masashi;Tsukiyama, Tadasuke;Hatakeyama, Shigetsugu
包含三分基序的蛋白质（TRIM）家族蛋白质参与广泛的生物过程，并且一致地，它们的改变导致不同的病理状况，例如遗传疾病、病毒感染和癌症发展。在这项研究中，我们发现TRIM家族蛋白之一TRIM 31在胃肠道中高度表达，并与信号转导子之一p52（Shc）相互作用。我们还通过结合测定发现，除了RING结构域之外的几乎整个区域是与p52（Shc）结合所必需的，但是通过脉冲追踪分析发现，TRIM 31的过表达不影响p52（Shc）的稳定性。此外，我们发现TRIM 31的过表达抑制了由活性形式的c-Src诱导的锚定非依赖性细胞生长。这些结果表明，TRIM 31减弱c-Src信号通过p52（Shc）在锚定非依赖性生长条件下，并可能与胃肠道细胞的生长活性。(C)2009爱思唯尔公司All rights reserved.
Tripartite motif-containing protein (TRIM) family proteins are involved in a broad range of biological processes and, consistently, their alterations result in diverse pathological conditions such as genetic diseases, viral infection and cancer development. In this study, we found that one of the TRIM family proteins, TRIM31, is highly expressed in the gastrointestinal tract and interacts with p52(Shc), one of the signal transducers. We also found by a binding assay that almost the whole region other than the RING domain is required for the binding to p52(Shc) but found by pulse-chase analysis that overexpression of TRIM31 does not affect the stability of p52(Shc). Moreover, we found that overexpression of TRIM31 suppresses anchorage-independent cell growth induced by the active form of c-Src. These results suggest that TRIM31 attenuates c-Src signaling via p52(Shc) under anchorage-independent growth conditions and is potentially associated with growth activity of cells in the gastrointestinal tract. (C) 2009 Elsevier Inc. All rights reserved.