Potentiation activity of multiple antibacterial agents by Salvianolate from the Chinese medicine Danshen against methicillin-resistant Staphylococcus aureus (MRSA)
Potentiation activity of multiple antibacterial agents by Salvianolate from the Chinese medicine Danshen against methicillin-resistant Staphylococcus aureus (MRSA)
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中药丹参丹参多酚酸盐对耐甲氧西林金黄色葡萄球菌（MRSA）的增强活性
DOI：
10.1016/j.jphs.2015.10.009
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发表时间：
2016-05-01
影响因子：
3.5
通讯作者：
Tang, Hua-Shu
中科院分区：
文献类型：
--
作者：
Liu, Qing-Qing;Han, Jun;Tang, Hua-Shu
丹参(Sal)是丹参的处方药。目前已广泛应用于冠心病等疾病的治疗，疗效显著。测定了丹参对感染病原菌的体外抗菌活性，并评价了其与10种抗生素对10株SCCmecⅢ型耐甲氧西林金黄色葡萄球菌(MRSA)的联合作用。用微量肉汤稀释法测定对每种药剂单独的敏感性，用棋盘实验和时间杀灭实验测定联合作用。结果表明，SAL对耐甲氧西林金黄色葡萄球菌的MIC值为128~256 mg/L。SAL/氨苄西林(磷霉素、红霉素、哌拉西林-他唑巴坦或克林霉素)对半数以上的分离菌有显著的协同作用，其最低抑菌浓度(MIC)分别降低了稀释倍数(TOD)至4-32(FICI为0.375~0.50)。SAL/AMP联合用药的抑菌、杀菌效果最好，SAL/AMK联合用药逆转了MRSA对AMK的耐药性。结果表明，SAL广泛增强了10种抗菌药的体外抗MRSA效果，有可能用于MRSA感染患者的联合治疗，值得进一步研究。(C)2015年提交人。由爱思唯尔B.V.代表日本药理学会制作和主办。这是CC BY-NC-ND License(http://creativecommons.org/licenses/by-nc-nd/4.0/).下的一篇开放获取文章
Salvianolate (SAL) is a prescribed medicine from the Chinese herb Danshen (Salvia miltiorrhiza Bunge). It has been widely used in treatment of coronary and other diseases with significant effects. The in vitro antimicrobial activities of SAL against infectious pathogens were assayed and its combined effects on 10 clinical isolates of SCCmec III type methicillin-resistant Staphylococcus aureus (MRSA) with ten antibiotics were evaluated. Susceptibility to each agent alone was tested using a broth microdilution method, and the chequerboard and time-kill experiments were used for the combined activities. The results showed MIC was 128-256 mg/L for SAL used alone against MRSA. Significant synergies were observed for SAL/Ampicillin (Fosfomycin, Erythromycin, Piperacillin-tazobactam or Clindamycin) combination against over half of the isolates, with their MICs reduced by times of dilution (TOD) to 4-32 (FICIs 0.375-0.5), respectively. SAL/AMP combination showed the best combined effect of synergy on bacteriostatic and bactericidal activities, while SAL/AMK combination reversed the resistance of MRSA to AMK. The results demonstrated that SAL enhanced widely the in vitro anti-MRSA efficacy of the ten antibacterial agents, which had potential for combinatory therapy of patients infected with MRSA and warrants further investigations. (C) 2015 The Authors. Production and hosting by Elsevier B. V. on behalf of Japanese Pharmacological Society. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).