Clonogenic hepatoblasts, common precursors for hepatocytic and biliary lineages, are lacking classical major histocompatibility complex class I antigen
Clonogenic hepatoblasts, common precursors for hepatocytic and biliary lineages, are lacking classical major histocompatibility complex class I antigen
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DOI：
10.1073/pnas.97.22.12132
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发表时间：
2000-10-24
影响因子：
11.1
通讯作者：
Reid, LM
中科院分区：
文献类型：
--
作者：
Kubota, H;Reid, LM
体外集落形成实验和流式细胞术鉴定大鼠成肝细胞为经典的MHC-I类，RT1A(I-)OX18(Low)细胞间黏附分子-1(ICAM-1)(+)。从单个RT1a(I-)祖细胞获得的大多数集落均可诱导向胆系分化，证明了它们的双向性。这些发现证明了克隆性肝母细胞的抗原性，并证明了它们的双能性。此外，成体肝细胞的克隆形成需要表皮生长因子，而没有表皮生长因子则促进了肝母细胞的克隆性生长。成体肝细胞集落由RT1A(I+)OX18(+)ICAM-1(++)细胞组成。这些结果表明，与具有明显增殖活性的成体肝细胞相比，成肝细胞具有独特的特征。成肝细胞的表型鉴定和克隆培养体系对于从成人中鉴定肝干细胞、研究肝脏发育以及基于肝祖细胞的细胞治疗具有重要意义。
An in vitro colony-forming assay and flow cytometry were used to identify rat hepatoblasts as being classical MHC class I, RT1A(I-)OX18(low) intercellular adhesion molecule 1 (ICAM-1)(+). Inducible differentiation toward biliary lineage was observed in most colonies derived from single RT1A(I-) progenitors, proving their bipotentiality, These findings demonstrate the antigenic profile of clonogenic hepatoblasts and proof of their bipotency, Furthermore, whereas colony formation of adult hepatocytes required epidermal growth factor, clonal growth of hepatoblasts was potentiated without epidermal growth factor. The adult hepatic colonies consisted of RT1A(I+)OX18(+)ICAM-1(++) cells. These results indicate that hepatoblasts possess unique characteristics as compared with adult hepatocytes harboring significant proliferative activity. The phenotypic identity of hepatoblasts and the clonal culture system have relevance for identifying hepatic stem cells from adults, for studying liver development, and for cell therapy based on hepatic progenitors.