A trans-zeatin riboside in root xylem sap negatively regulates adventitious root formation on cucumber hypocotyls
A trans-zeatin riboside in root xylem sap negatively regulates adventitious root formation on cucumber hypocotyls
复制标题
DOI：
10.1093/jxb/erf077
复制
发表时间：
2002-11-01
影响因子：
6.9
通讯作者：
Satoh, S
中科院分区：
文献类型：
--
作者：
Kuroha, T;Kato, H;Satoh, S
以黄瓜地上部培养为材料，分析了根衍生物质在下胚轴组织不定根形成中的作用。西葫芦根部木质部汁液对下胚轴不定根的形成有较强的抑制作用。双溶剂提取、正反相柱层析分离和液相色谱/串联质谱联用分析表明，反式玉米素核苷(ZR)是不定根形成的主要抑制因子。ZR是木质部汁液中存在的主要细胞分裂素，浓度为2×10~(-8)M时，浓度为3.16×10~(-9)M的ZR抑制不定根的形成。这些结果表明，通过木质部汁液从根中运输的ZR可能是植物下胚轴不定根形成的内源抑制因子。
Shoot cultures of cucumber were used to analyse the roles of root-derived substances in adventitious root formation on hypocotyl tissues. Xylem sap collected from the roots of squash had a strong inhibitory effect on the formation of hypocotyl adventitious roots. Double-solvent extraction followed by fractionation with both normal and reverse phase column chromatographies and analysis by liquid chromatography/tandem mass spectrometry identified transzeatin riboside (ZR) as the primary suppressor of adventitious root formation. ZR was the predominant cytokinin present in the xylem sap, occurring at a concentration of 2x10(-8) M. Application of ZR at concentrations from 3.16x10(-9) M effected inhibition of adventitious root formation. These results suggest that ZR transported from roots via xylem sap may act as an endogenous suppressor of hypocotyl adventitious root formation in planta.