Use of Calcium Antagonism for the Characterization of Drug‐Evoked Dopamine Release from the Brain of Conscious Rats Determined by Microdialysis
Use of Calcium Antagonism for the Characterization of Drug‐Evoked Dopamine Release from the Brain of Conscious Rats Determined by Microdialysis
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利用钙拮抗作用表征通过微透析测定的清醒大鼠大脑中药物诱发的多巴胺释放
DOI：
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发表时间：
1989
影响因子：
4.7
通讯作者：
J. B. Vries
中科院分区：
文献类型：
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作者：
B. Westerink;R. M. Hofsteede;J. Tuntler;J. B. Vries
摘要：采用清醒大鼠纹状体微透析法测定多巴胺含量。我们研究了9种不同药物治疗诱导的多巴胺释放是否对钙拮抗敏感。通过Mg2+或Cd2+输注测定钙拮抗作用。研究了以下条件：氟哌啶醇、氟哌啶醇加GBR 12909、诺米芬、（+）‐安非他明（均腹腔注射）、KC1、l‐甲基‐4‐苯基‐吡啶离子（MPP+）、谷氨酸、瓦巴因和120 mmol/ l镁（均通过透析膜输注）。钙拮抗结果与河豚毒素（TTX）敏感性数据相结合。通过综合数据，表征了三种不同类型的多巴胺释放。首先，用生理盐水、氟哌啶醇、氟哌啶醇加GBR 12909、诺米芬和瓦巴因治疗的动物观察动作电位依赖性多巴胺释放。其次，在（+）‐安非他明、谷氨酸、MPP+和120 mmol/L Mg2+的情况下，动作电位独立释放被建立。最后，K+诱导的多巴胺释放被分类为TTX独立和钙依赖。结论：脑透析是区分不同类型神经递质释放的有效方法。
Abstract: Dopamine was determined by microdialysis of the striatum of conscious rats. We investigated whether the release of dopamine, induced by nine different pharmacological treatments, was sensitive to calcium antagonism. Calcium antagonism was determined by Mg2+ or Cd2+ infusion. The following conditions were investigated: haloperidol, haloper‐idol plus GBR 12909, nomifensine, (+)‐amphetamine (all administered intraperitoneally), KC1, l‐methyl‐4‐phenyl‐pyridinium ion (MPP+), glutamate, ouabain, and 120 mmol/ L magnesium (all applied by infusion through the dialysis membrane). The results on calcium antagonism were combined with data on tetrodotoxin (TTX) sensitivity. With the combined data, three different types of dopamine release were characterized. First, action potential‐dependent dopamine release was observed in animals treated with saline, haloperidol, haloperidol plus GBR 12909, nomifensine, and ouabain. Second, action potential‐independent release was established in the case of (+)‐amphetamine, glutamate, MPP+, and 120 mmol/L Mg2+. Finally, K+‐induced dopamine release was classified as TTX independent and calcium dependent. It is concluded that brain dialysis is a powerful method for differentiating between different types of neurotransmitter release.
脑线粒体能量驱动的 N-甲基-4-苯基吡啶摄取介导 MPTP 的神经毒性。
影响因子：
6.1
作者：
Ramsay,RR;Dadgar,J;Trevor,A;Singer,TP
通讯作者：
Singer,TP
影响因子：
56.9
作者：
MILLER, RJ
通讯作者：
MILLER, RJ