Reconstruction and Morphometric Analysis of Hippocampal Neurons from Mice Expressing Fluorescent Proteins
Reconstruction and Morphometric Analysis of Hippocampal Neurons from Mice Expressing Fluorescent Proteins
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DOI：
10.1007/978-1-4939-0381-8_12
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发表时间：
2014-01-01
期刊：
影响因子：
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通讯作者：
Bakota, Lidia
中科院分区：
文献类型：
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作者：
Golovyashkina, Nataliya;Suendermann, Frederik;Bakota, Lidia
树突形态及其改变决定了单个神经元集成信号的强度。树突状树枝化的变化伴随着神经元发育、记忆形成和神经退行性过程。区域特异性树突简化是阿尔茨海默病和其他神经退行性疾病的一个关键特征。在这里，我们描述了一种分析从不同神经元亚群中表达增强型绿色荧光蛋白（EGFP）的转基因小鼠制备的脑切片中的全神经元形态的方法。使用高分辨率共聚焦显微镜对完整的锥体神经元进行成像，并在半自动 3D 重建后进行分析。该方法可以评估不同的形态特征，例如树突的总范围和分支点的数量，以及 3D Sholl 分析，例如来自不同海马或皮质亚区域的神经元的顶端和基底树突子树。
Dendritic morphology and its alterations determine the strength of the integrated signal of individual neurons. Changes in the dendritic arborization accompany neuronal development, memory formation, and neurodegenerative processes. Region-specific dendritic simplification is a key feature in Alzheimer's disease and other neurodegenerative disorders. Here we describe a method to analyze whole-neuron morphology in brain slices prepared from transgenic mice expressing enhanced green fluorescent protein (EGFP) in different subpopulations of neurons. Complete pyramidal neurons are imaged using high-resolution confocal microscopy and analyzed after semi-automated 3D reconstruction. The approach allows evaluation of different morphological features, such as total extent of dendrites and number of branching points, as well as 3D Sholl analysis, e.g., of apical and basal dendritic subtrees of neurons from different hippocampal or cortical subregions.