Photopolymerized poly(ethylene glycol)/poly(L-lysine) hydrogels for the delivery of neural progenitor cells
Photopolymerized poly(ethylene glycol)/poly(L-lysine) hydrogels for the delivery of neural progenitor cells
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DOI：
10.1163/156856207781494368
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发表时间：
2007-08-01
影响因子：
3.6
通讯作者：
Lavik, Erin B.
中科院分区：
文献类型：
--
作者：
Hynes, Sara Royce;Mcgregor, Lynn M.;Lavik, Erin B.
神经祖细胞（npc）在许多疾病和损伤模型中显示出前景，但为了使这些细胞安全有效，它们必须在移植后适当地分化。我们已经开发了一种由聚乙二醇（PEG）与聚l -赖氨酸（PLL）结合的大分子组成的光聚合水凝胶，它支持NPC存活并指导分化。绿色荧光蛋白（GFP）阳性的npc被包裹在这些凝胶中，并显示存活长达17天。当光引发剂浓度为5.0 mg/ml时，很少有npc（0.5 +/- 0.25%）发生凋亡。此外，55 +/- 6%的npc在含有表皮生长因子（EGF）的培养基中培养成成熟的神经细胞类型（神经丝200阳性），而其余44 +/- 8%未分化（巢蛋白阳性）。一小部分（1 +/- 0.4%）表达星形胶质细胞标记物胶质酸性原纤维蛋白。光聚合PEG/PLL凝胶可促进npc的存活并指导其分化，使该系统成为治疗中枢神经系统损伤和疾病的npc的一种有前景的递送载体。
Neural progenitor cells (NPCs) have shown promise in a number of models of disease and injury, but for these cells to be safe and effective, they must be directed to differentiate appropriately following transplantation. We have developed a photopolymerized hydrogel composed of macromers of poly(ethylene glycol) (PEG) bound to poly(L-lysine) (PLL) that supports NPC survival and directs differentiation. Green fluorescent protein (GFP) positive NPCs were encapsulated in these gels and demonstrated survival up to 17 days. When encapsulated in the gels at a photoinitiator concentration of 5.0 mg/ml, few NPCs (0.5 +/- 0.25%) demonstrated apoptosis. Furthermore, 55 +/- 6% of the NPCs cultured within the gels in epidermal growth factor (EGF) containing media differentiated into a mature neuronal cell type (neurofilament 200 positive) while the remainder 44 +/- 8% were undifferentiated (nestin positive). A small percentage, 1 +/- 0.4%, expressed the astrocytic marker glial acidic fibrilary protein. Photopolymerized PEG/PLL gels promote the survival and direct the differentiation of NPCs, making this system a promising delivery vehicle for NPCs in the treatment of injuries and diseases of the central nervous system.