ANTISENSE PROBING OF THE HUMAN U4/U6 SNRNP WITH BIOTINYLATED 2'-OME RNA OLIGONUCLEOTIDES
ANTISENSE PROBING OF THE HUMAN U4/U6 SNRNP WITH BIOTINYLATED 2'-OME RNA OLIGONUCLEOTIDES
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DOI：
10.1016/0092-8674(89)90036-6
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发表时间：
1989-11-03
期刊：
影响因子：
64.5
通讯作者：
LAMOND, AI
中科院分区：
文献类型：
--
作者：
BLENCOWE, BJ;SPROAT, BS;LAMOND, AI
We have used antisense 2''-OMe RNA oligonucleotides carrying four 5''-terminal biotin residues to probe the structure and function of the human U4/U6 snRNP. Nine oligonucleotides, complementary to multiple regions of U4 and U6 snRNAs, bound stably and specifically to U4/U6 snRNP. This allowed for efficient and selective removal of U4/U6 from HeLa cell nuclear extracts. Binding of oligonucleotides to certain snRNA domains inhibited splicing and affected the U4-U6 interaction. Pre-mRNA and splicing products could also be affinity-selected through binding of the oligonucleotides to U4/U6 snRNPs in splicing complexes. The results suggest that U4 snRNP is not released during spliceosome assembly.
