Proposal of screening method for intestinal mucus adhesive lactobacilli using the enzymatic activity of glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Proposal of screening method for intestinal mucus adhesive lactobacilli using the enzymatic activity of glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
复制标题
DOI：
10.1111/j.1740-0929.2012.01054.x
复制
发表时间：
2013-01-01
影响因子：
2
通讯作者：
Saito, Tadao
中科院分区：
文献类型：
--
作者：
Kinoshita, Hideki;Imoto, Shun;Saito, Tadao
粘附测试是复杂的，耗时的和昂贵的，而益生菌乳酸杆菌的最重要的标准是粘附到人体肠道的能力。用微量滴定板筛选法测定了30株人肠道乳酸杆菌的细胞表面甘油醛-3-磷酸脱氢酶（GAPDH）活性。30份培养12 h的样品中有21份检测到GAPDH活性，18 h的样品全部检测到GAPDH活性。这表明GAPDH在许多乳酸杆菌的细菌细胞表面普遍表达。BIACORE粘附试验显示GAPDH活性与粘附之间存在统计学显著的正相关性（P
Adhesion tests are complex, time-consuming and expensive, while the most important criterion for a probiotic lactobacilli is the ability to adhere to the human intestine. Thirty lactobacilli isolates from human intestinal tissues were measured for cell surface glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity using a microtiter plate screening method. GAPDH activities were detected in 21 out of 30 samples from 12h cultures and in all samples from 18h cultures. This suggests GAPDH is universally expressed on the bacterial cell surfaces from many lactobacilli. A statistically significant positive correlation was shown between GAPDH activity and adhesion using the BIACORE adhesion assay (P