THE INDEPENDENT STAGE-SPECIFIC EXPRESSION OF THE 18-KDA HEAT-SHOCK PROTEIN GENES DURING MICROSPOROGENESIS IN ZEA-MAYS L
THE INDEPENDENT STAGE-SPECIFIC EXPRESSION OF THE 18-KDA HEAT-SHOCK PROTEIN GENES DURING MICROSPOROGENESIS IN ZEA-MAYS L
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DOI：
10.1002/dvg.1020140104
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发表时间：
1993-01-01
期刊：
影响因子：
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通讯作者：
WALDEN, DB
中科院分区：
文献类型：
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作者：
ATKINSON, BG;RAIZADA, M;WALDEN, DB
玉米的小分子热激蛋白（18-kDa）是由一个复杂的多基因家族编码的。在以前的报告中，我们描述了编码该家族两个不同成员的cDNA的遗传信息。在这次交流中，我们报告的分离和表征的cDNA和基因组克隆编码信息的第三个成员的热休克蛋白家族（c/gMHSP 18 -1）。制备含有这些表征的18-kDa基因中的每一个共有或特异的核苷酸序列的DNA片段，并将其用作探针以评估这些基因在玉米近交系（Oh 43）中的小孢子发生和配子体发育期间的表达。我们的研究结果表明：（1）mRNA转录编码的18-kDa的热休克蛋白的表达和/或积累在小孢子发生过程中，和（2）基因编码的两个特征的18-kDa的热休克蛋白的表达和/或积累独立的，在一个阶段特异性的方式在小孢子发生。这些观察结果表明，特定的18 kDa热休克蛋白基因的阶段特异性表达的结果，从基因特异性调节过程中的小孢子发生和配子体发育，而不是从整体激活的热休克或应激反应。
The small (18-kDa) heat shock proteins (hsps) of maize are encoded by a complex multigene family. In a previous report, we described the genetic information from cDNAs encoding two different members of the family. In this communication, we report the isolation and characterization of cDNA and genomic clones encoding information for a third member of this hsp family (c/gMHSP18-1). DNA fragments containing nucleotide sequences common to, or specific for, each of these characterized 18-kDa genes were prepared and used as probes to assess the expression of these genes during microsporogenesis and development of the gametophyte in an inbred line of maize (Oh43). Our results demonstrate (1) that mRNA transcripts encoding the 18-kDa hsps are expressed and/or accumulate during microsporogenesis, and (2) that genes encoding two of the characterized 18-kDa hsps are expressed and/or accumulate independently, in a stage-specific manner during microsporogenesis. These observations imply that the stage-specific expression of particular 18-kDa hsp genes results from gene-specific regulation during microsporogenesis and gametophyte development rather than from an overall activation of the heat shock or stress response.