Relationship between the nicotinamide adenine dinucleotide redox potential and the 2,3-diphosphoglycerate content in the erythrocyte in sickle cell disease.
Relationship between the nicotinamide adenine dinucleotide redox potential and the 2,3-diphosphoglycerate content in the erythrocyte in sickle cell disease.
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镰状细胞病中烟酰胺腺嘌呤二核苷酸氧化还原电位与红细胞中2,3-二磷酸甘油酸含量的关系。
DOI：
10.1111/j.1365-2141.1989.tb07693.x
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发表时间：
1989
影响因子：
6.5
通讯作者：
Tanaka,KR
中科院分区：
文献类型：
--
作者：
Lachant,NA;Zerez,CR;Tanaka,KR
镰状细胞病患者红细胞(RBC)中氧化形式的烟酰胺腺嘌呤二核苷酸(NAD+/(NAD+和NADH)；即NAD+/NADT比值)百分比增加。我们验证了这一假设，即NAD+/NADT比值的增加是SCD红细胞2，3-二磷酸甘油酸酯(DPG)含量增加的决定因素。在正常人和镰状细胞病或自身免疫性溶血性贫血患者中，观察到红细胞压积与红细胞DPG浓度呈负相关(r=0.69)，红细胞NAD+/NADT比值与−浓度呈正相关(r=0.74)。经协方差分析剔除PCV对DPG的影响后，NAD+/NADT比值与DPGadj(PCV)呈显著正相关(r=0·50，P<0·001)。在体外培养研究中，增加NAD+/NADT比值显著增加正常和AIHA红细胞的DPG含量。相反，降低NAD+/NADT比值会降低正常、AIHA和SCD红细胞的DPG含量。因此，SCD红细胞中DPG含量的增加似乎部分是由于NAD+/NADT比率的增加，而不仅仅是对携氧能力下降的生理反应。
The percentage of nicotinamide adenine dinucleotide (NAD) in the oxidized form [NAD+/(NAD+and NADH); i.e. the NAD+/NADTratio] is increased in the red cell (RBC) in sickle cell disease. We tested the hypothesis that the increased NAD+/NADTratio was a determinant of the increased 2,3‐diphosphoglycerate (DPG) content of the SCD RBC. Using normal subjects and individuals with sickle cell disease or autoimmune haemolytic anaemia (AIHA), we observed an inverse relationship between the packed cell volume (PCV) and the RBC DPG concentration (r=−0·69) and a direct relationship between the RBC NAD+/NADTratio and the DPG concentration (r= 0·74). When the effect of the PCV on DPG was removed using analysis of covariance [DPGady(PCV)], the NAD+/NADTratio had a significant relationship with the DPGadj(PCV)(r= 0·50,P< 0·001). Inin vitroincubation studies, increasing the NAD+/NADTratio significantly increased the DPG content of both normal and AIHA RBC. Conversely, decreasing the NAD+/NADTratio decreased the DPG content of normal, AIHA and SCD RBC. Thus, the increased DPG content in the SCD RBC appears to be due, in part, to the increased NAD+/NADTratio and is not purely a physiologic response to decreased oxygen carrying capacity.