Liposome-Encapsulated Rec8 and Dmrt1 Plasmids Induce Red-Spotted Grouper (Epinephelus akaara) Testis Maturation
Liposome-Encapsulated Rec8 and Dmrt1 Plasmids Induce Red-Spotted Grouper (Epinephelus akaara) Testis Maturation
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DOI：
10.1007/s10126-022-10111-5
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发表时间：
2022-03
影响因子：
3
通讯作者：
Xuzhuo Duan;Xianze Jia;K. Liang;Fengqi Huang;Jinhong Shan;Huitao Chen;Xinhe Ruan;Lihua Li;Huihong Zhao;Qing Wang
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文献类型：
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作者：
Xuzhuo Duan;Xianze Jia;K. Liang;Fengqi Huang;Jinhong Shan;Huitao Chen;Xinhe Ruan;Lihua Li;Huihong Zhao;Qing Wang
在鱼类中，性腺的成熟在种群的发育和繁殖中起着重要的作用，它也决定了圈养繁殖的成功。因此，寻找促进性腺成熟的方法是水产养殖的重要目标。本研究将脂质体包装的重组dmrt 1和rec8过表达质粒分别注射到赤点石斑鱼（Epineodorus akaara）未成熟精巢中，检测Dmrt 1和Rec8蛋白在体内的表达。性腺指数（GSI）和性腺组织学分析表明，睾丸发育从未成熟到成熟状态在7天内质粒注射后。此外，精子的浓度和活力在注入质粒的鱼是相同的自然成熟的鱼。这些结果为dmrt 1和rec8表达质粒经睾丸内注射诱导睾丸成熟提供了证据。
In fish, the maturity of gonads plays an important role in the development and reproduction of the population, and it also dictates the success of captive breeding. Therefore, finding ways to promote gonadal maturation is an important goal in aquaculture. In this study, we injected recombinantdmrt1andrec8overexpression plasmids packaged in liposomes into the immature testis of red-spotted grouper (Epinephelus akaara) and measured the expression of Dmrt1 and Rec8 protein in vivo. Gonadosomatic index (GSI) and gonadal histology analyses showed that the testis developed from the immature to the mature state within 7 days after plasmid injection. Additionally, the spermatozoa concentration and motility in plasmid-injected fish was the same as that of naturally mature fish. These results provided evidence that delivery ofdmrt1andrec8expression plasmids into the testis via injection induced testis maturation in vivo.