The effect of 1,25(OH)2D3 on alkaline phosphatase in osteoblastic osteosarcoma cells.
The effect of 1,25(OH)2D3 on alkaline phosphatase in osteoblastic osteosarcoma cells.
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DOI：
10.1016/s0021-9258(18)34781-1
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发表时间：
1982-04
期刊：
影响因子：
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通讯作者：
R. Majeska;G. Rodan
中科院分区：
文献类型：
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作者：
R. Majeska;G. Rodan
1,25-二羟基维生素D3对生长、细胞蛋白和碱性磷酸酶的影响在不同表型成熟阶段的克隆成骨样大鼠骨肉瘤细胞系的培养中进行了研究，以碱性磷酸酶活性水平为标准。在碱性磷酸酶特异性活性较低的“未成熟”培养物中，1,25-二羟基维生素D3在10(-8)-10(-7)M时抑制细胞生长并提高碱性磷酸酶活性；较低的激素浓度没有影响。在碱性磷酸酶活性较高的培养物中，激素具有双相效应：在10(-8)-10(-7)M浓度下，1,25-二羟基维生素D3抑制生长并升高碱性磷酸酶和细胞总蛋白；然而，较低的激素浓度（10（-10）和10(-9)M）降低了碱性磷酸酶的活性。这种效果在更“成熟”的培养中更为明显，在10(-11)m附近，碱性磷酸酶活性的抑制达到一半。在这个阶段，激素引起细胞数量的少量增加。数据表明，1,25-二羟基维生素D3对骨骼细胞的影响取决于它们的生长或成熟状态。这一发现可能为1,25-二羟基维生素D3对骨骼的影响提供新的见解，并可能有助于解决关于激素对培养成骨样细胞影响的明显相互矛盾的实验结果。
The effects of 1,25-dihydroxyvitamin D3 on growth, cell protein, and alkaline phosphatase were examined in cultures of a clonal osteoblast-like rat osteosarcoma cell line at different stages of phenotypic maturation, defined by the level of alkaline phosphatase activity. In "immature" cultures with low alkaline phosphatase specific activity, 1,25-dihydroxyvitamin D3 at 10(-8)-10(-7) M inhibited cell growth and elevated alkaline phosphatase activity; lower hormone concentrations had no effect. In cultures with higher alkaline phosphatase activity, the hormone had biphasic effects: at concentrations of 10(-8)-10(-7) M, 1,25-dihydroxyvitamin D3 inhibited growth and elevated alkaline phosphatase and total cell protein; however, lower hormone concentrations (10(-10) and 10(-9) M) reduced alkaline phosphatase activity. This effect was more pronounced in more "mature" cultures where half-maximum inhibition of alkaline phosphatase activity occurred near 10(-11) M. At this stage, the hormone caused a small increase in cell number. The data suggest that 1,25-dihydroxyvitamin D3 exerts differential effects on skeletal cells depending on their state of growth or maturation. This finding may offer new insights into the effects of 1,25-dihydroxyvitamin D3 on bone and may help resolve apparently conflicting experimental findings on the effects of the hormone on osteoblast-like cells in culture.