01-P012 The role of micro-RNAs in the development of the chick gonads
01-P012 The role of micro-RNAs in the development of the chick gonads
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01-P012 micro-RNAs在雏鸡性腺发育中的作用
DOI：
10.1016/j.mod.2009.06.013
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发表时间：
2009
影响因子：
2.6
通讯作者：
McBride D
中科院分区：
文献类型：
--
作者：
McBride D
micro-RNA（miRNA）是一类在转录后水平调控基因表达的小分子RNA。目前已知有数百种miRNA序列，它们在多细胞植物和动物中高度保守。许多是组织特异性的，并且已知调节发育事件。我们已经启动了一个项目，以确定参与小鸡性腺发育的miRNAs。我们从发育中的卵巢和睾丸中分离的RNA中产生并测序了miRNA文库。我们鉴定了大量不同的miRNAs，其中大约40%是以前在任何物种中都没有报道过的全新miRNAs。在雄性和雌性文库中比较每个miRNA序列的观察频率。正如预期的那样，鉴定的所有miRNA中的大多数（95%）在男性和女性文库中以相似的水平存在，其余的以性二态的方式表达：在男性或女性中高2至19倍。我们认为这些性二态性miRNAs参与了性腺的结构和功能发育。已经获得了所有性二态miRNAs在整个性腺发育过程中的详细表达谱，我们目前正在通过消除发育中的雄性和雌性鸡胚中内源性miRNAs的水平来评估这些miRNAs的作用。
Micro-RNAs (miRNA) represent a large class of small RNAs that regulate gene expression at the post-transcriptional level. Several hundred miRNA sequences are now known and are highly conserved in multicellular plants and animals. Many are tissue specific and are known to regulate developmental events. We have initiated a project to identify miRNAs involved in gonadal development in the chick. We generated and sequenced miRNA libraries from RNA isolated from the developing ovaries and testes. We identified a large number of different miRNAs, of which approximately 40% are entirely novel miRNAs that have not been previously reported in any species. The frequency of observation of each miRNA sequence was compared in male and female libraries. As expected, the majority (95%) of all miRNAs identified are present at similar levels in male and female libraries, with the remainder expressed in a sexually dimorphic fashion: between 2 and 19 fold higher in either males or females. We believe that these sexually dimorphic miRNAs are involved in the structural and functional development of the gonads. Detailed expression profiles throughout gonadal development have been obtained for all sexually dimorphic miRNAs and we are currently assessing the roles of these miRNA by depleting levels of endogenous miRNAs in developing male and female chick embryos.