Isolation of tick-borne encephalitis virus from wild rodents and a seroepizootiologic survey in Hokkaido, Japan
Isolation of tick-borne encephalitis virus from wild rodents and a seroepizootiologic survey in Hokkaido, Japan
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DOI：
10.4269/ajtmh.1999.60.287
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发表时间：
1999-02-01
影响因子：
3.3
通讯作者：
Takashima, I
中科院分区：
文献类型：
--
作者：
Takeda, T;Ito, T;Takashima, I
为了确定日本北海道南部地区蜱传脑炎（TBE）病毒的脊椎动物宿主，利用该地区捕获的小哺乳动物的脾脏进行了病毒分离。分离到两株病毒，一株来自特纹姬蚊，另一株来自红绒螯虾。将病毒分离株接种于幼鼠肾细胞单层，制备抗原载玻片，用于间接免疫荧光抗体测定。两株分离株经单克隆抗体反应鉴定为TEE病毒。为了确定北海道的tbe流行区，对1992年至1997年收集的啮齿动物、马和狗的血清进行了中和抗体检测，以对抗先前从狗身上分离的TEE病毒。阳性病例分布在北海道南部4个区。
To determine the vertebrate host of tick-borne encephalitis (TBE) virus in the southern part of Hokkaido, Japan, virus isolation was performed using spleens from small mammals captured in the area. Two virus strains were isolated, one strain from Apodemus speciosus and another from Clethrionomys rufocanus. Virus isolates were inoculated onto baby hamster kidney cell monolayers and antigen slides were prepared for an indirect immunofluorescent antibody assay. Two isolates were identified as TEE viruses by monoclonal antibody reactions. To specify the TBE-endemic area in Hokkaido, rodent, horse, and dog sera collected from 1992 to 1997 were tested for neutralization antibody against TEE virus previously isolated from a dog. The positive cases were distributed in four districts in the southern part of Hokkaido.