Visual detection of pandemic influenza A H1N1 Virus 2009 by reverse-transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye
Visual detection of pandemic influenza A H1N1 Virus 2009 by reverse-transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye
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DOI：
10.1016/j.jviromet.2010.03.027
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发表时间：
2010-08-01
影响因子：
3.1
通讯作者：
Dong, Xiao-ping
中科院分区：
文献类型：
--
作者：
Ma, Xue-jun;Shu, Yue-long;Dong, Xiao-ping
建立了一种灵敏的逆转录环介导等温扩增（RT-LAMP）方法，用于快速检测甲型流感Hi - NI病毒感染。该方法在65℃、60 mm的单管中一步完成反应，扩增前加入羟基酚蓝（HNB）染料，RI-LAMP检测限约为60个拷贝。该方法在50例临床诊断为季节性流感或甲型大流行性流感H1N1病毒感染的临床标本中进一步进行了评估，结果表明，RT-LAMP与HNB染料是一种快速检测甲型大流行性流感Hi - NI病毒(C)的灵敏简便的方法
A sensitive reverse-transcription loop-mediated Isothermal amplification (RT-LAMP) assay was developed for rapid visual detection of pandemic influenza A Hi NI virus infection The reaction was performed in one step in a single tube at 65 degrees C for 60 mm with the addition of hydroxynaphthol blue (HNB) dye prior to amplification The detection limit of the RI-LAMP assay was approximately 60 copies, and no cross-detection was observed The assay was evaluated further with 50 clinical specimens diagnosed clinically with seasonal influenza or pandemic influenza A H1N1 virus infection RT-LAMP with HNB dye was demonstrated to be a sensitive and easy assay for rapid detection of pandemic influenza A Hi NI virus (C)2010 Elsevier B V All rights reserved