Bright AIE Nanoparticles with F127 Encapsulation for Deep-Tissue Three-Photon Intravital Brain Angiography
Bright AIE Nanoparticles with F127 Encapsulation for Deep-Tissue Three-Photon Intravital Brain Angiography
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DOI：
10.1002/adhm.201700685
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发表时间：
2017-11-08
影响因子：
10
通讯作者：
Qian, Jun
中科院分区：
文献类型：
--
作者：
Wang, Yalun;Han, Xiao;Qian, Jun
深部组织成像在生物学应用中具有重要意义。本文合成了一种具有聚集诱导发光（AIE）特性的深红色发光材料2，3-二（4 ′-（二苯基氨基）-[1，1 ′-联苯]-4-基）苯甲腈（TPATCN）。然后将TPATCN分子包封在普朗尼克F-127的聚合物基质中以形成纳米颗粒（NP）。TPATCN纳米粒在深红色区域显示明亮的三光子荧光（3 PF），同时具有高的化学稳定性、良好的光稳定性和生物相容性。在1550 nm飞秒激光的激发下，它们进一步用于小鼠脑血管系统的体内3 PF成像。一个生动的3D重建的脑血管系统，然后建立与穿透深度为875 μ m，这是有史以来最大的基于AIE纳米粒子的3 PF成像。之后，通过采集3 PF和三次谐波产生信号，进一步实现脑血管的多通道非线性光学成像。这些结果将有助于进一步研究脑的结构和功能。
Deep-tissue imaging is of great significance to biological applications. In this paper, a deep-red emissive luminogen 2,3-bis(4'-(diphenylamino)-[1,1'-biphenyl]- 4-yl) fumaronitrile (TPATCN) with aggregation-induced emission (AIE) feature is prepared. TPATCN molecules were then encapsulated within a polymeric matrix of Pluronic F-127 to form nanoparticles (NPs). TPATCN NPs exhibit bright three-photon fluorescence (3PF) in deep-red region, together with high chemical stability, good photostability, and biocompatibility. They are further utilized for in vivo 3PF imaging of the brain vasculature of mice, under the excitation of a 1550 nm femtosecond laser. A vivid 3D reconstruction of the brain vasculature is then built with a penetration depth of 875 mu m, which is the largest in ever reported 3PF imaging based on AIE NPs. After that, by collecting both of the 3PF and third-harmonic generation signals, multichannel nonlinear optical imaging of the brain blood vessels is further realized. These results will be helpful to study the structures and functions of the brain in the future.