A method for the reconstruction of multifocal structured illumination microscopy data with high efficiency
A method for the reconstruction of multifocal structured illumination microscopy data with high efficiency
复制标题
一种高效重建多焦点结构照明显微镜数据的方法
DOI：
10.1038/s41598-019-49762-1
复制
发表时间：
2019-09-16
影响因子：
4.6
通讯作者：
Liu, Weiwei
中科院分区：
文献类型：
--
作者：
Feng, Liang;Zhou, Langfeng;Liu, Weiwei
我们提出并证明了一种有效的方法，利用几个原始图像的多焦点结构照明显微镜（MSIM）获得的配置文件的重建。首先，我们提出一种方法来产生九个原始的多焦点图像与增强调制深度，以实现均匀照明的样品。然后，结合阵列的参数，逐行逐列地执行结构照明显微镜（SIM）的标准结构重建过程。最后，我们将这些复原影像联合收割机组合在一起，以获得具有增强的分辨率和良好对比度的最终影像。理论分析和数值模拟表明，该方法在MSIM数据图像重建领域显示出巨大的潜力。
We present and demonstrate an efficient method for the reconstruction of profiles acquired by multifocal structured illumination microscopy (MSIM) utilizing few raw images. Firstly, we propose a method to produce nine raw multifocal images with enhanced modulation depth to accomplish the uniform illumination of the sample. Then, combing with the parameter of the arrays, we perform the standard construct reconstruction procedure of structured illumination microscopy (SIM) row by row and column by column. Finally, we combine these restored images together to obtain the final image with enhanced resolution and good contrast. Based on theoretical analysis and numerical simulations, this method shows great potential in the field of the image reconstruction of MSIM data.