Highly polymerized procyanidins in brown soybean seed coat with a high radical-scavenging activity
Highly polymerized procyanidins in brown soybean seed coat with a high radical-scavenging activity
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DOI：
10.1021/jf010307x
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发表时间：
2001-12-01
影响因子：
6.1
通讯作者：
Suda, I
中科院分区：
文献类型：
--
作者：
Takahata, Y;Ohnishi-Kameyama, M;Suda, I
研究了来自褐大豆品种 Akita-Zairai 种皮的 70% 丙酮水提取物的 1,1-二苯基-2-三硝基苯肼 (DPPH) 自由基清除活性。秋田在来种皮活性远高于其他三个红棕色品种，但低于两个黑色品种，并且与酚类化合物的含量密切相关。在 Akita-Zairai 的 LH20 柱色谱中，在用 MeOH 和 70% 丙酮水溶液洗脱的级分中检测到高 DPPH 自由基清除活性。在具有高自由基清除活性的级分中也检测到了原花青素。基质辅助激光解吸/电离飞行时间质谱分析表明，棕色或黑色大豆种皮中所含原花青素的聚合度(DP)高达DP30。
1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity of the 70% aqueous acetone extract from the seed coat of the brown soybean variety, Akita-Zairai, was investigated. The activity of the seed coat of Akita-Zairai was much higher than that of three other reddish-brown varieties, but lower than that of two black varieties, and was closely dependent on the content of phenolic compounds. In the LH20 column chromatography of Akita-Zairai, high DPPH radical-scavenging activities were detected in the fractions eluted with MeOH and 70% aqueous acetone. Proanthocyanidins were also detected in fractions showing high radical-scavenging activities. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry analysis showed that the degree of polymerization (DP) of the procyanidins contained in the brown or black soybean seed coat was as high as DP30.