LYMPHOCYTES FROM MULTIPLE-SCLEROSIS PATIENTS PRODUCE ELEVATED LEVELS OF GAMMA-INTERFERON INVITRO
LYMPHOCYTES FROM MULTIPLE-SCLEROSIS PATIENTS PRODUCE ELEVATED LEVELS OF GAMMA-INTERFERON INVITRO
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DOI：
10.1007/bf00915335
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发表时间：
1985-11-01
影响因子：
9.1
通讯作者：
JOHNSON, KP
中科院分区：
文献类型：
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作者：
HIRSCH, RL;PANITCH, HS;JOHNSON, KP
对多发性硬化（MS）患者和对照组的单个核细胞（MNC）产生γ干扰素（IFN）进行了评价。用伴刀豆球蛋白A培养3天后，通过放射免疫测定法测定上清液中γ IFN的量。MS患者的MNC产生的γ-IFN显著高于正常对照或其他神经系统疾病患者的MNC（P < 0.001）。还测量了血清和CSF中γ IFN的水平。尽管血清中γ IFN相对缺乏（30例中4例阳性），但所有检测的CSF样本均具有较低但可检测的γ IFN水平（0.3至1.4 U/ml）。这些研究表明，MS中的一些自身免疫特征和免疫异常可能与γ IFN产生升高有关。
The production of gamma interferon (IFN) by mononuclear cells (MNC) from patients with exacerbating/remitting multiple sclerosis (MS) and controls was evaluated. After 3 days of culture with concanavalin A, the amount of gamma IFN in supernatant fluids was determined by radioimmunoassay. MNC from MS patients produced significantly (P < 0.001) more gamma IFN than MNC from either normal controls or patients with other neurologic diseases. Levels of gamma IFN in the serum and CSF were also measured. Despite the relative absence of gamma IFN in serum (4 positive of 30), all CSF samples tested had low, but detectable, levels of gamma IFN (0.3 to 1.4 U/ml). These studies suggest that some of the autoimmune features and immunologic abnormalities in MS may be related to elevated gamma IFN production.