1,25-dihydroxyvitamin D3 and prostaglandin E2 act directly on circulating human osteoclast precursors
1,25-dihydroxyvitamin D3 and prostaglandin E2 act directly on circulating human osteoclast precursors
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DOI：
10.1006/bbrc.1999.1545
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发表时间：
1999-10-22
影响因子：
3.1
通讯作者：
Athanasou, NA
中科院分区：
文献类型：
--
作者：
Itonaga, I;Sabokbar, A;Athanasou, NA
已知 1,25-二羟基维生素 D-3 (1,25(OH)(2)D-3) 和前列腺素 E-2 (PGE(2)) 通过对成骨细胞的作用间接影响破骨细胞的形成。为了确定 1,25(OH)(2)D-3 和 PGE(2) 是否也对循环破骨细胞前体有直接影响，在骨保护素配体和巨噬细胞集落刺激因子 (M-CSF)（+/- 地塞米松）存在下，将这些因子添加到人外周血单核细胞 (PBMC) 的长期培养物中。当单独添加1,25(OH)(2)D-3时，TRAP(+)和VNR+多核细胞的数量和腔隙吸收面积减少。当将 1,25(OH)(2)D-3 和地塞米松组合添加到 PBMC 培养物中时，注意到吸收坑形成显着增加。当在存在或不存在地塞米松的情况下将 PGE(2) 添加到 PBMC 培养物中时，可以观察到破骨细胞形成和腔隙吸收的剂量依赖性抑制。因此，1,25(OH)(2)D-3 和 PGE(2) 不仅影响骨基质细胞存在下的破骨细胞形成，而且还直接作用于循环破骨细胞前体，影响破骨细胞分化。 (C) 1999 年学术出版社。
1,25-Dihydroxyvitamin D-3 (1,25(OH)(2)D-3) and prostaglandin E-2 (PGE(2)) are known to influence osteoclast formation indirectly through their effects on osteoblasts. To determine whether 1,25(OH)(2)D-3 and PGE(2) also have a direct effect on circulating osteoclast precursors, these factors were added to long-term cultures of human peripheral blood mononuclear cells (PBMCs) in the presence of osteoprotegerin ligand and macrophage colony-stimulating factor (M-CSF) (+/-dexamethasone). The number of TRAP(+) and VNR+ multinucleated cells and the area of lacunar resorption were decreased when 1,25(OH)(2)D-3 alone was added. A marked increase in resorption pit formation was noted when the combination of 1,25(OH)(2)D-3 and dexamethasone was added to PBMC cultures. Dose-dependent inhibition of osteoclast formation and lacunar resorption was seen when PGE(2) was added to PBMC cultures in both the presence and the absence of dexamethasone. Thus, 1,25(OH)(2)D-3 and PGE(2) not only influence osteoclast formation in the presence of bone stromal cells but also act directly on circulating osteoclast precursors to influence osteoclast differentiation. (C) 1999 Academic Press.