Polymorphism of the Prolactin Gene and Its Relationship with Milk Production in Gir and Kankrej Cattle.
Polymorphism of the Prolactin Gene and Its Relationship with Milk Production in Gir and Kankrej Cattle.
复制标题
DOI：
10.4103/jnsbm.jnsbm_303_16
复制
发表时间：
2017-07
期刊：
影响因子：
--
通讯作者：
Chauhan JB
中科院分区：
文献类型：
--
作者：
Patel JB;Chauhan JB
本研究旨在检测Gir（200）和Kankrej（100）牛催乳素（PRL）基因的等位基因频率和基因型频率，并分析催乳素基因型牛的产奶性状。采用聚合酶链反应-限制性片段长度多态性技术扩增位于外显子3的156 bp片段。等位基因频率在研究品种A = 0.52和B = 0.48。在Gir牛中，AA基因型的产奶量和脂肪含量（%）的平均值和标准差分别为3811.6 ± 462.1和3.99 ± 0.18，AB基因型的产奶量和脂肪含量（%）的平均值和标准差分别为3514.9 ± 450.8和4.16 ± 0.33，BB基因型的产奶量和脂肪含量的平均值和标准差分别为3388.8 ± 423.3和4.34 ± 0.11。同样，对于Kankrej牛，AA基因型的产奶量和脂肪含量（%）的平均值和标准差分别为2007.8 ± 246.3和4.05 ± 0.16，AB基因型为1846.14 ± 133.4和4.10 ± 0.156，BB基因型为1767.7 ± 186.4和4.30 ± 0.178，在康克瑞牛身上。本研究表明，泌乳素基因型的Gir和Kankrej牛之间的牛奶性状的差异。
The aim of this study was to detect allelic and genotypic frequencies of prolactin (PRL) gene in Gir (200) and Kankrej (100) cattle and to analyze milk production traits of tested cattle on their PRL genotypes. The 156 bp fragment located in exon 3 was amplified using polymerase chain reaction-restriction fragments length polymorphism technique. Allele frequencies in the studied breed were A = 0.52 and B = 0.48. Means and standard deviations for milk yield and fat content (%) were 3811.6 ± 462.1 and 3.99 ± 0.18 for the AA genotype, 3514.9 ± 450.8 and 4.16 ± 0.33 for the AB genotype, and 3388.8 ± 423.3 and 4.34 ± 0.11 for the BB genotype, respectively, in Gir cattle. Similarly, for Kankrej cattle, means and standard deviations for milk yield and fat content (%) were 2007.8 ± 246.3 and 4.05 ± 0.16 for the AA genotype, 1846.14 ± 133.4 and 4.10 ± 0.156 for the AB genotype, and 1767.7 ± 186.4 and 4.30 ± 0.178 for the BB genotype, respectively, in Kankrej cattle. This study showed differences in milk traits among PRL genotypes of Gir and Kankrej cattle.