Cytosolic and microsomal epoxide hydrolases are immunologically distinguishable from each other in the rat and mouse.
Cytosolic and microsomal epoxide hydrolases are immunologically distinguishable from each other in the rat and mouse.
复制标题
大鼠和小鼠中的胞质环氧化物水解酶和微粒体环氧化物水解酶在免疫学上是可区分的。
DOI：
10.1016/s0021-9258(19)69582-7
复制
发表时间：
1981
期刊：
影响因子：
--
通讯作者：
F. Oesch
中科院分区：
文献类型：
--
作者：
T. Guenthner;B. Hammock;U. Vogel;F. Oesch
用同种大鼠肝微粒体环氧化物水解酶的抗体区分微粒体环氧化物水解酶与小鼠和大鼠胞质来源的环氧化物水解酶。使用琼脂糖凝胶双扩散分析，我们表明，抗大鼠肝微粒体环氧化物水解酶形成一个单一的沉淀线与大鼠和小鼠肝脏的溶解微粒体，但没有反应被视为与相应的胞质组分。大鼠或小鼠微粒体环氧化物水解酶活性（使用苯并[a]芘4，5-氧化物作为底物）可以通过抗体从溶解的制剂中完全沉淀出来，该抗体对大鼠和小鼠微粒体环氧化物水解酶具有等效性。使用任何浓度的检测抗体均未观察到胞质水解酶活性沉淀（使用反式-β-乙基氧化苯乙烯作为底物）。因此，在微粒体环氧化物水解酶的情况下，大鼠和小鼠这两个物种之间存在广泛的免疫交叉反应性。相比之下，没有交叉反应性是检测胞质和微粒体环氧化物水解酶之间，甚至当酶从相同的物种进行比较。我们的结论是微粒体和胞浆环氧化物水解酶活性代表不同的和免疫学上的非交叉反应蛋白质种类。
Antibodies raised to homogeneous rat liver microsomal epoxide hydrolase were used to distinguish microsomal epoxide hydrolase from epoxide hydrolase of cytosolic origin in mice and rats. Using double diffusion analysis in agarose gels, we show that anti-rat liver microsomal epoxide hydrolase forms a single precipitin line with solubilized microsomes from rat and mouse liver, but no reaction is seen with the corresponding cytosolic fractions. Rat or mouse microsomal epoxide hydrolase activity (using benzo[a]pyrene 4,5-oxide as substrate) can be completely precipitated out of solubilized preparations by the antibody, which is equipotent against rat and mouse microsomal epoxide hydrolase. No precipitation of cytosolic hydrolase activity (using trans-beta-ethyl styrene oxide as substrate) is seen with any concentration of the antibody tested. Thus, in the case of microsomal epoxide hydrolase, extensive immunological cross-reactivity exists between the two species, rat and mouse. In contrast, no cross-reactivity is detectable between cytosolic and microsomal epoxide hydrolase, even when enzymes from the same species are compared. We conclude that microsomal and cytosolic epoxide hydrolase activities represent distinct and immunologically non-cross-reactive protein species.