Effects of low temperature and abscisic acid on the expression of the sorbitol-6-phosphate dehydrogenase gene in apple leaves
Effects of low temperature and abscisic acid on the expression of the sorbitol-6-phosphate dehydrogenase gene in apple leaves
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DOI：
10.2503/jjshs.75.20
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发表时间：
2006-01-01
影响因子：
--
通讯作者：
Yamaki, S
中科院分区：
文献类型：
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作者：
Kanayama, Y;Watanabe, M;Yamaki, S
山梨醇-6-磷酸脱氢酶（S6 PDH）是蔷薇科果树光合作用早期产物山梨醇生物合成的关键酶。研究了低温和脱落酸（阿坝）对苹果叶片S6 PDH基因表达的影响。在叶盘实验中，S6 PDH的表达增强阿坝处理，以及低温和高盐胁迫。S6 PDH mRNA的表达水平在阿坝处理后8h开始上升，在10 μ M阿坝处理下，S6 PDH mRNA的表达水平最高。结果表明，随着温度的降低，果园叶片中S6 PDH mRNA的表达量增加，阿坝含量增加。这种诱导可能部分是对低温的应激反应，这是在铸造冬季耐冻性的先决条件。Southern杂交分析表明，S6 PDH基因在苹果基因组中是一个单拷贝基因，它是一个独特的多功能基因，在各种逆境条件下参与山梨醇的合成，并参与光合产物的转运。
Sorbitol-6-phosphate dehydrogenase (S6PDH) is known to be a key enzyme in the biosynthesis of sorbitol, an early product of photosynthesis, common to fruit trees of the Rosaceae family. Effects of low temperature and abscisic acid (ABA) on the expression of the S6PDH gene were investigated in apple leaves. In leaf-disc experiments, the expression of S6PDH was enhanced by an ABA treatment, as well as by low temperature and high-salinity stresses. The level of S6PDH mRNA increased 8 h after the addition of ABA: the highest level of S6PDH mRNA resulted on exposure to 10 mu M ABA. The level of S6PDH mRNA in leaves of apple trees growing in an orchard increased with a decrease in temperature in the Call while ABA content increased. This induction may partly be a stress-response to low temperature, a prerequisite for freezing tolerance during the coining winter. Southern blot analysis revealed that S6PDH is a single-copy gene in the apple genome, indicating that it is a unique, multifunctional one, for sorbitol biosynthesis under various stress responses, as well as for the translocation of photosynthates.