Identification and initial characterization of transforming growth factor-like mitogen(s) in human anterior pituitary.
Identification and initial characterization of transforming growth factor-like mitogen(s) in human anterior pituitary.
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人类垂体前叶转化生长因子样有丝分裂原的鉴定和初步表征。
DOI：
10.1016/0006-291x(85)91228-8
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发表时间：
1985
影响因子：
3.1
通讯作者：
Yasuo Imai
中科院分区：
文献类型：
--
作者：
Toshio Tsushima;Y. Ohba;Naoya Emoto;Kazuo Shizume;Yasuo Imai
转化生长因子（TGF）是一个肽家族，通过其诱导非肿瘤指示细胞在软琼脂中非贴壁依赖性生长的能力来定义。我们发现人垂体前叶组织的酸乙醇提取物能够刺激正常大鼠肾成纤维细胞在软琼脂中的集落生长。当在1 M乙酸中在Bio-Gel P-60柱上进行凝胶过滤时，大部分TGF活性洗脱在对应于表观摩尔重量15，000的级分中。该活性是热和酸稳定的，但通过用胰蛋白酶和二硫苏糖醇处理而失活。PGF-like材料不与表皮生长因子（EGF）竞争受体结合，也不需要EGF的集落形成活性。因此，人垂体TGF与α、β TGF不同。
Transforming growth factor (TGFs) are a family of peptide(s) defined by their ability to induce anchorage-independent growth of non-neoplastic indicator cells in soft agar. We found that acid-ethanol extracts of human anterior pituitary tissues were able to stimulate colony growth of normal rat kidney fibroblasts in soft agar. When subjected to gel-filtration on a column of Bio-Gel P-60 in 1 M acetic acid, the majority of TGF activity eluted in fractions corresponding to an apparent mol wt 15,000. The activity was heat- and acid-stable, but was inactivated by treatment with trypsin and dithiothreitol. Pituitary TGF-like materials did not compete with epidermal growth factor (EGF) for receptor binding and did not require EGF for colony-forming activity. Thus, human pituitary TGF was not like type α or type β TGF.
人类实体恶性肿瘤中的转化生长因子。
影响因子：
11.2
作者：
Nickell,KA;Halper,J;Moses,HL
通讯作者：
Moses,HL