The Determination of Triglycerides in Plasma and Tissues
The Determination of Triglycerides in Plasma and Tissues
复制标题
血浆和组织中甘油三酯的测定
DOI：
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发表时间：
1968
期刊：
影响因子：
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通讯作者：
J. Manning
中科院分区：
文献类型：
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作者：
V. Sardesai;J. Manning
本文介绍了一种测定血浆和组织甘油三酯的简便方法。该过程包括甘油三酯的提取和皂化，甘油部分氧化为甲醛，甲醛转化为黄色化合物3，5-二乙酰基-1-4-二氢卢利啶，其强度通过比色法测定。添加至血浆和组织中的甘油三酯的回收率令人满意。发现从正常人受试者获得的血浆样品具有83-200 mg范围内的甘油三酯。100毫升从灵敏度、简单性和所需时间的角度来看，该技术被认为是对先前描述的甘油三酯测定程序的改进。
A simple method for the determination of plasma and tissue triglycerides is described. This procedure involves the extraction and saponification of triglycerides, the oxidation of the glycerol moiety to formaldehyde, and the conversion of formaldehyde to a yellow-colored compound, 3,5 diacetyl-1-4 dihydrolulidine, the intensity of which is determined spectrophotometrically. The recoveries of triglycerides added to plasma and tissues have been satisfactory. Plasma samples obtained from normal human subjects are found to have triglycerides in the range 83-200 mg./100 ml. From the standpoint of sensitivity, simplicity, and time required, this technic is believed to be an improvement over previously described procedures for triglyceride determination.