Development of a novel method for specific detection of genetically modified Atlantic salmon, AquAdvantage, using real-time polymerase chain reaction
Development of a novel method for specific detection of genetically modified Atlantic salmon, AquAdvantage, using real-time polymerase chain reaction
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使用实时聚合酶链反应开发一种特异性检测转基因大西洋鲑鱼的新方法 AquAdvantage
DOI：
10.1016/j.foodchem.2019.125426
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发表时间：
2020
期刊：
影响因子：
8.8
通讯作者：
Kondo Kazunari
中科院分区：
文献类型：
--
作者：
Soga Keisuke;Nakamura Kosuke;Ishigaki Takumi;Kimata Shinya;Ohmori Kiyomi;Kishine Masahiro;Mano Junichi;Takabatake Reona;Kitta Kazumi;Nagoya Hiroyuki;Kondo Kazunari
转基因大西洋鲑鱼AquAdvantage（AquAd）是第一种正式批准供人类食用的转基因动物。许多国家根据其转基因法规监测该产品的使用，但需要一个实用的AquAd特异性检测系统。在这里，我们开发了一种高灵敏度的实时聚合酶链反应方法，用于检测食品中的AquAd。该方法显示出对AquAd转基因的高特异性，并且检测限为每个测试反应12.5-25个靶向DNA拷贝。使用开发的方法进行的实验室间研究证明了在>0.1%（w/w）AquAd含量下的重现性。
Genetically modified (GM) Atlantic salmon, AquAdvantage (AquAd), was the first GM animal approved officially for human consumption. Many countries monitor the use of this product under their GM regulations, but a pragmatic system for AquAd-specific detection is needed. Here, we developed a real-time polymerase chain reaction method with high sensitivity for detection of AquAd in foods. This method showed high specificity for the AquAd transgene and the detection limit was 12.5–25 targeted DNA copies per test reaction. An inter-laboratory study using the method developed demonstrated reproducibility at >0.1% (w/w) AquAd content.