Pancreatic lipase-related protein 2 is essential for egg hatching in the brown planthopper, Nilaparvata lugens
Pancreatic lipase-related protein 2 is essential for egg hatching in the brown planthopper, Nilaparvata lugens
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胰脂肪酶相关蛋白 2 对于褐飞虱 (Nilaparvata lugens) 卵的孵化至关重要
DOI：
10.1111/imb.12290
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发表时间：
2017-06-01
影响因子：
2.6
通讯作者：
Bao, Y. -Y.
中科院分区：
文献类型：
--
作者：
Xu, L.;Huang, H. -J.;Bao, Y. -Y.
在这项研究中，我们确定了一个胰腺脂肪酶相关蛋白2（PLRP 2）基因，通过搜索转录组数据的褐飞虱，褐飞虱，单食性水稻害虫。PLRP2 mRNA首先在人胰腺中分离，并且在来自饮食的半乳糖脂的水解中起重要作用。虽然在许多昆虫物种中已经鉴定出同源PLRP 2基因，但其生理功能仍然未知。本研究首次报道了PLRP 2在昆虫物种中的功能作用。与哺乳动物PLRP 2s不同，N. Lugens PLRP 2在雌性成虫卵巢发育中的卵母细胞中高度表达，而在产卵中几乎不表达。抑制N. Lugens PLRP2的RNA干扰表达显著抑制水稻幼苗卵的孵化，表明N. Lugens PLRP 2对卵母细胞的成熟和发育具有重要作用。这一新的发现将提高我们对昆虫生殖策略的理解，并为未来的作物害虫管理提供潜在的目标。
In this study, we identified a pancreatic lipase-related protein 2 (PLRP2) gene through searching the transcriptome data of the brown planthopper, Nilaparvata lugens, a monophagous rice pest. PLRP2 mRNAs were first isolated in the human pancreas and play an important role in hydrolysis of galactolipids from the diet. Although homologous PLRP2 genes have been identified in many insect species, their physiological functions remain unknown. The present study for the first time reports the functional role of PLRP2 in an insect species. Differing from mammal PLRP2s, N. lugens PLRP2 was highly expressed in developing oocytes of the ovaries of female adults and little expressed in laid eggs. Suppression of N. lugens PLRP2 expression using RNA interference significantly inhibited egg hatching in rice seedlings, implying that N. lugens PLRP2 was important for oocyte maturation and development. This novel finding will improve our understanding of the reproductive strategies in insects and provides a potential target for future management of crop pests.