Normal range of complement components during pregnancy: A prospective study
Normal range of complement components during pregnancy: A prospective study
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怀孕期间补体成分的正常范围：一项前瞻性研究
DOI：
10.1111/aji.13202
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发表时间：
2019-11-12
影响因子：
3.6
通讯作者：
Zhao, Ming-hui
中科院分区：
文献类型：
--
作者：
He, Ying-dong;Xu, Bing-ning;Zhao, Ming-hui
问题 补体系统在正常胎盘形成中发挥着关键作用，补体系统激活的精细调节对于成功妊娠至关重要。因此，建立妊娠期补体成分的正常范围对于临床评估和研究具有重要意义。方法我们进行了一项前瞻性研究，调查妊娠不同阶段循环中补体成分的正常范围。使用免疫比浊法测量补体因子 B (CFB)、C1q、补体因子 H (CFH)、C3、C3c 和 C4 的血浆浓度；通过酶联免疫吸附测定（ELISA）测定妊娠不同时间点的甘露聚糖结合凝集素（MBL）、C3a、C5a和可溶性C5b-9（sC5b-9）水平。结果 共采集正常妊娠女性362例血浆样本733份，非孕妇血浆样本65份。在妊娠前三个月，CFB、CFH、MBL、C3c、C4 和 C3a 的水平分别为 414.5 +/- 85.9 mg/L（平均值 95% CI：402.4-426.6 mg/L）、381.0 +/- 89.0 mg/L（平均值 95% CI：368.5-393.6 mg/L）、 4274.5 +/- 2752 ng/mL（平均值的 95% CI：3881.1-4656.4 ng/mL）、1346.9 +/- 419.8 mg/L（平均值的 95% CI：1287.7-1406.0 mg/L）、357.4 +/- 101.8 mg/L（平均值的 95% CI： 343.0-371.7 mg/L) 和 182.5 +/- 150.0 ng/mL（平均值的 95% CI：186.9-229.1 ng/mL）。 C3和C4的水平在整个怀孕期间逐渐增加。妊娠早期和中期的 C1q、C5a 和 sC5b-9 水平与非妊娠女性几乎相同。结论本研究结果表明妊娠本身可能影响补体系统成分的血浆水平。
Problem The complement system plays a key role in normal placentation, and delicate regulation of complement system activation is critical for successful pregnancy. Therefore, establishing a normal range of complement components during pregnancy is important for clinical evaluation and research. Methods We performed a prospective study to investigate the normal range of complement components in circulation during different stages of pregnancy. Plasma concentrations of complement factor B (CFB), C1q, complement factor H (CFH), C3, C3c, and C4 were measured using an immunoturbidimetric assay; mannan-binding lectin (MBL), C3a, C5a, and soluble C5b-9 (sC5b-9) levels at different time points of pregnancy were determined by enzyme-linked immunosorbent assay (ELISA). Results A total of 733 plasma samples were collected from 362 women with a normal pregnancy and 65 samples from non-pregnant women. In the first trimester of pregnancy, the levels of CFB, CFH, MBL, C3c, C4, and C3a were 414.5 +/- 85.9 mg/L (95% CI for mean: 402.4-426.6 mg/L), 381.0 +/- 89.0 mg/L (95% CI for mean: 368.5-393.6 mg/L), 4274.5 +/- 2752 ng/mL (95% CI for mean: 3881.1-4656.4 ng/mL), 1346.9 +/- 419.8 mg/L (95% CI for mean: 1287.7-1406.0 mg/L), 357.4 +/- 101.8 mg/L (95% CI for mean: 343.0-371.7 mg/L), and 182.5 +/- 150.0 ng/mL (95% CI for mean: 186.9-229.1 ng/mL), respectively. The levels of C3 and C4 increased gradually throughout pregnancy. The levels of C1q, C5a, and sC5b-9 in the first and second trimesters were nearly the same as those in non-pregnant women. Conclusion The results of this study show that pregnancy itself may influence the plasma levels of complement system components.