Regulatory B cells in mouse models of intestinal inflammation.
Regulatory B cells in mouse models of intestinal inflammation.
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小鼠肠道炎症模型中的调节性 B 细胞。
DOI：
10.1007/978-1-4939-1161-5_16
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Mizoguchi,Atsushi
中科院分区：
文献类型：
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作者：
Nishida,Atsushi;Lau,CindyW;Mizoguchi,Emiko;Mizoguchi,Atsushi
炎症性肠病（IBD）是一种慢性肠道炎症性疾病，其发病率和患病率在世界范围内不断增加。尽管人们普遍认为 B 细胞由于自身抗体的产生而在 IBD 中发挥致病作用，但来自小鼠模型的越来越多的证据表明，致病性 B 细胞和称为 Breg 的调节性 B 细胞在这种疾病中共存。由于需要一些独特的技术来密切研究肠道相关淋巴组织（GALT）中的 Breg，因此我们在此描述如何在小鼠中诱导结肠炎以及如何分析 GALT 特异性 Breg 的表型和功能。
Inflammatory bowel disease (IBD) is a chronic intestinal inflammatory condition with increasing incidence and prevalence around the world. Although B cells had generally been believed to play a pathogenic role in IBD due to the production of autoantibodies, a growing body of evidence from mouse models suggests the coexistence of pathogenic B cells and regulatory B cells, termed Breg, in this disorder. Since some unique techniques are required to closely study the Breg in gut-associated lymphoid tissues (GALT), we herein describe how to induce colitis in mice and how to analyze the phenotype and function of GALT-specific Breg.