Methods for determining protein translocation by the Legionella pneumophila Dot/Icm type IV secretion system.
Methods for determining protein translocation by the Legionella pneumophila Dot/Icm type IV secretion system.
复制标题
通过嗜肺军团菌 Dot/Icm IV 型分泌系统测定蛋白质易位的方法。
DOI：
10.1007/978-1-62703-161-5_19
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Luo,Zhao-Qing
中科院分区：
文献类型：
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作者：
Zhu,Wenhan;Luo,Zhao-Qing
嗜肺军团菌是军团病的病原体，主动操纵宿主细胞过程，建立一个允许其复制的膜结合复制液泡。这种复制生态位的建立需要Dot/Icm IV型分泌系统，该系统可将大量效应物转运到宿主细胞中。确定一个特定的蛋白质是否是转运体的底物是后续功能研究的先决条件。因此，在过去的十年中，已经开发了多种方法来测量Dot/ icm依赖性蛋白质进入宿主细胞的递送。这些方法和适当的筛选策略的结合已经允许鉴定270多个易位蛋白。这些工作为进一步研究这些蛋白在l和l之间相互作用中的作用奠定了坚实的基础。嗜肺菌及其宿主。在这里，我们总结了这些方法的实验细节。
Legionella pneumophilathe causative agent of Legionnaires’ disease, actively manipulates host cell processes to establish a membrane-bound replication vacuole permissive for its replication. Establishment of such replication niche requires the Dot/Icm type IV secretion system which translocates a plethora of effectors into host cells. Determining whether a particular protein is a substrate of the transporter is a prerequisite for subsequent functional studies. Thus, a variety of methods have been developed in the last decade to measure Dot/Icm-dependent delivery of protein into the host cell. The combination of these methods and the appropriate screening strategies has allowed for the identification of more than 270 translocated proteins. These efforts have laid a solid foundation for further study of the roles of these proteins in the interactions betweenL. pneumophilaand its host. Here, we summarized the experimental details of these methods.