Urethane dimethacrylate induces cytotoxicity and regulates cyclooxygenase-2, hemeoxygenase and carboxylesterase expression in human dental pulp cells
Urethane dimethacrylate induces cytotoxicity and regulates cyclooxygenase-2, hemeoxygenase and carboxylesterase expression in human dental pulp cells
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DOI：
10.1016/j.actbio.2013.10.006
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发表时间：
2014-02-01
影响因子：
9.7
通讯作者：
Jeng, Jiiang-Huei
中科院分区：
文献类型：
--
作者：
Chang, Hsiao-Hua;Chang, Mei-Chi;Jeng, Jiiang-Huei
二甲基丙烯酸氨基甲酸乙酯（UDMA）是一种主要的牙科树脂单体，其对人类牙髓的毒性作用尚不完全清楚。本研究探讨了UDMA对牙髓细胞的细胞毒性、细胞周期分布、凋亡及相关基因表达的影响。研究了活性氧、血红素加氧酶-1 （HO-1）和羧酸酯酶（CES）在UDMA细胞毒性中的作用。在0.1 ~ 0.35 mM浓度下，UDMA诱导牙髓细胞形态改变，使牙髓细胞活力降低29 ~ 49%，诱导G0/G1、G2/M细胞周期阻滞和凋亡。UDMA可抑制cdc2、cyclinB1和cdc25C的表达。UDMA刺激髓细胞COX-2、HO-1和CES2 mRNA的表达。n-乙酰- l-半胱氨酸、过氧化氢酶和酯酶能减弱UDMA的细胞毒性，而zn -原卟啉（HO-1抑制剂）、BNPP （CES抑制剂）和洛哌丁胺（CES2抑制剂）能增强UDMA的细胞毒性。UDMA暴露可能诱发牙髓的炎症和毒性。这些发现对于了解牙髓修复和牙髓盖盖后树脂单体对牙髓的临床反应具有重要意义，也为牙材料的改进提供了线索。(C) 2013材料学报Elsevier Ltd.出版。版权所有。
The toxic effect of urethane dimethacrylate (UDMA), a major dental resin monomer, on human dental pulp is not fully clear. In this study, we investigated the influence of UDMA on the cytotoxicity, cell cycle distribution, apoptosis and related gene expression of dental pulp cells. The role of reactive oxygen species, hemeoxygenase-1 (HO-1) and carboxylesterase (CES) in UDMA cytotoxicity, was evaluated. UDMA induced morphological changes of pulp cells and decreased cell viability by 29-49% at concentrations of 0.1-0.35 mM. UDMA induced G0/G1, G2/M cell cycle arrest and apoptosis. The expression of cdc2, cyclinB1 and cdc25C was inhibited by UDMA. Moreover, UDMA stimulated COX-2, HO-1 and CES2 mRNA expression of pulp cells. The cytotoxicity of UDMA was attenuated by N-acetyl-L-cysteine, catalase and esterase, but was enhanced by Zn-protoporphyrin (HO-1 inhibitor), BNPP (CES inhibitor) and loperamide (CES2 inhibitor). Exposure of UDMA may potentially induce the inflammation and toxicity of dental pulp. These findings are important for understanding the clinical response of human pulp to resin monomers after operative restoration and pulp capping, and also provide clues for improvement of dental materials. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.