Regulation of Zinc Transporter 1 Expression in Dorsal Horn of Spinal Cord After Acute Spinal Cord Injury of Rats by Dietary Zinc
Regulation of Zinc Transporter 1 Expression in Dorsal Horn of Spinal Cord After Acute Spinal Cord Injury of Rats by Dietary Zinc
复制标题
膳食锌对大鼠急性脊髓损伤后脊髓背角锌转运蛋白1表达的调节
DOI：
10.1007/s12011-012-9414-9
复制
发表时间：
2012-11-01
影响因子：
3.9
通讯作者：
Zhang, Li
中科院分区：
文献类型：
--
作者：
Su, Ribao;Mei, Xifan;Zhang, Li
脊髓背角中的锌浓度对于伤口愈合、神经功能和生殖是重要的。然而，在脊髓损伤（SCI）后的大鼠中，脊髓对饮食锌改变的反应是未知的。本研究探讨了不同锌饮食对脊髓损伤后脊髓背角细胞锌水平和锌转运蛋白1（ZnT 1）表达的影响。将144只雄性Wistar大鼠随机分为4组：假手术组（30 mg Zn/kg）、高锌饮食SCI模型组（ZH，180 mg Zn/kg）、锌充足饮食SCI模型组（30 mg Zn/kg）和边缘缺锌饮食SCI模型组（MZD，5 mg Zn/kg）。为了验证这一假设，饮食锌可能调节急性脊髓损伤后脊髓背角ZnT 1表达的作用，我们跟踪ZnT 1蛋白和锌离子免疫组化，蛋白质印迹，和automographics。ZH组术后脊髓背角锌和ZnT 1水平显著升高（P < 0.05），术后第7天达高峰（P < 0.05），随后开始下降。但MZD组脊髓锌含量和ZnT 1表达均降低（P < 0.05）。ZnT 1蛋白表达与脊髓锌含量呈正相关（R = 0.49880，P = 0.0492）。我们发现锌和ZnT 1在脊髓中的表达受饮食锌的调节。上述结果提示，膳食锌可能对脊髓损伤后脊髓背角ZnT 1的表达有调节作用。同时，ZnT 1可能在SCI中锌稳态的维持中发挥重要作用。
Zinc concentrations in the dorsal horn of spinal cord are important for wound healing, neurological function, and reproduction. However, the response of the spinal cord to alterations in dietary zinc is unknown in rats after spinal cord injury (SCI). The current study explored cellular zinc levels and zinc transporter 1 (ZnT1) expression in the dorsal horn of spinal cord with different dietary zinc after SCI. A hundred and forty-four male Wistar rats were randomly divided into four groups: sham-operated group (30 mg Zn/kg), zinc-high dietary SCI model group (ZH, 180 mg Zn/kg), zinc-adequate dietary SCI model group (30 mg Zn/kg), and marginal zinc-deficient dietary SCI model group (MZD, 5 mg Zn/kg). To test the hypothesis that dietary zinc may regulate role of ZnT1 expression in dorsal horn after acute SCI, we traced ZnT1 proteins and zinc ions with immunohistochemistry, western blot, and autometallography. Zinc and ZnT1 levels of the dorsal horn in ZH significantly increased after surgery (P < 0.05), reached peak level (P < 0.05) on the seventh day, and subsequently levels of their expression began to decrease. But zinc levels and ZnT1 expression of spinal cord in MZD dietary groups decreased (P < 0.05) in SCI. There was a positive correlation between ZnT1 protein and zinc content in spinal cord (R = 0.49880, P = 0.0492). We found that both zinc and ZnT1 expressions in spinal cord are regulated by dietary zinc. These results indicate that dietary zinc may regulate the expression of ZnT1 in the dorsal horn of spinal cord after SCI. ZnT1 may, at the same time, play a significant role in the maintenance of zinc homeostasis in SCI.