FvZFP1 confers transgenic Nicotiana benthamiana resistance against plant pathogens and improves tolerance to abiotic stresses
FvZFP1 confers transgenic Nicotiana benthamiana resistance against plant pathogens and improves tolerance to abiotic stresses
复制标题
FvZFP1 赋予转基因本塞姆氏烟草对植物病原体的抗性并提高对非生物胁迫的耐受性
DOI：
10.1016/j.plantsci.2021.111176
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发表时间：
2022-01-05
期刊：
影响因子：
5.2
通讯作者：
Jiang, Tong
中科院分区：
文献类型：
--
作者：
Rui, Penghuan;Yang, Xianchu;Jiang, Tong
锌指蛋白可以诱导植物对恶劣环境条件的抗性，激活抗性通路相关分子的表达。在此之前，我们发现了一种新的草莓花萼锌指蛋白与草莓静脉带病毒编码的P6蛋白相互作用。然而，锌指蛋白在植物抗逆性中的分子机制尚不清楚。在本研究中，我们报道了含环指和CHY锌指结构域蛋白1 （FvZFP1）的鉴定和功能表征。FvZFP1基因在烟叶中的过表达增强了烟叶花叶病毒（TMV）和丁香假单胞菌的抗性。番茄DC3000 （Pst DC3000）感染后ROS含量增加。此外，FvZFP1过表达上调了TMV和Pst DC3000感染后水杨酸（SA）反应相关基因的表达以及SA的积累。此外，FvZFP1过表达通过提高SOD活性和降低MDA含量提高了耐盐性和抗旱性。在盐度或干旱条件下，FvZFP1的过表达也激活了ABA通路。据我们所知，这是第一个关于牛皮囊藻锌指蛋白参与生物和非生物胁迫信号通路串扰的研究，表明FvZFP1是提高多重胁迫抗性的候选基因。
Zinc finger proteins can induce plant resistance and activate the expression of molecules involved in the resistance pathway in response to harsh environmental conditions. Previously, we found that a novel Fragaria vesca zinc finger protein interacts with the P6 protein encoded by a strawberry vein banding virus. However, the molecular mechanism of the zinc finger protein in plant stress resistance is still unknown. In this study, we reported the identification and functional characterization of the RING finger and CHY zinc finger domain containing protein 1 (FvZFP1). The overexpression of FvZFP1 in Nicotiana benthamiana enhanced resistance to tobacco mosaic virus (TMV) and Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) infection by increasing ROS content. Additionally, FvZFP1 overexpression upregulated salicylic acid (SA) response-related gene expression as well as SA accumulation following TMV and Pst DC3000 infection. Furthermore, FvZFP1 over expression resulted in increased salinity and drought stress tolerance by increasing SOD activity and decreasing MDA content. Overexpression of FvZFP1 also activated the ABA pathway under salinity or drought conditions. To our knowledge, this is the first study on the involvement of F. vesca zinc finger protein in crosstalk between biotic and abiotic stress signaling pathways, suggesting that FvZFP1 is a candidate gene for the improvement of resistance in response to multiple stresses.