Semi-automatic size measurement of primary particles in aggregated nanomaterials by transmission electron microscopy
Semi-automatic size measurement of primary particles in aggregated nanomaterials by transmission electron microscopy
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DOI：
10.1016/j.powtec.2014.04.040
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发表时间：
2014-07-01
影响因子：
5.2
通讯作者：
Mast, Jan
中科院分区：
文献类型：
--
作者：
De Temmerman, Pieter-Jan;Verleysen, Eveline;Mast, Jan
透射电子显微镜成像和半自动图像分析的组合允许检测和测量聚集的纳米材料的初级颗粒。在欧盟委员会纳米材料定义的框架内确定了粉末纳米材料的中值初级颗粒尺寸、基于数量的尺寸分布、纳米颗粒的百分比和体积比表面积。聚集的二氧化钛代表性测试纳米材料NM-100，平均初级粒径接近100 nm限值。基于分水岭分割检测初级粒子，并基于欧氏距离图测量其最小尺寸和重叠系数。NM-100的主要颗粒含有22%的纳米颗粒，其尺寸在1 nm和100 nm之间。该方法被评估用于测量其他聚集的TiO 2合成无定形二氧化硅和炭黑纳米材料，其中纳米颗粒的百分比接近100%。高水平的自动化允许有效地测量最大内切圆的直径。最大的内切圆是一个措施的最小初级颗粒尺寸在一个维度上，并示出与Feret分钟测量是可互换的。确定分形性质和分形状聚集体的体积比表面积的标准方法是细化支持的最大内切圆的直径和重叠系数测量每个单独的初级颗粒。(C)2014爱思唯尔有限公司版权所有。
A combination of transmission electron microscopic imaging and semi-automatic image analysis allows detecting and measuring the primary particles of aggregated nanomaterials. The median primary particle size, the number based size distribution, the percentage of nanoparticles and the volume specific surface area of powdered nanomaterials are determined in the framework of the European Commission's nanomaterial definition.The principle of a semi-automated approach to measure the minimal size in one dimension of primary particles in aggregates by transmission electron microscopy is developed using the model of the powdered, aggregated titanium dioxide representative test nanomaterial NM-100 with a mean primary particle diameter near the 100 nm limit. The primary particles are detected based on watershed segmentation and their minimal size and overlap coefficient are measured based on an Euclidean distance map. The primary partides of NM-100 contain 22% nanoparticles, with a size between 1 nm and 100 nm. The method is evaluated to measure other aggregated TiO2 synthetic amorphous silica and carbon black nanomaterials for which the percentage of nanoparticles is nearly 100%. A high level of automation allows measuring the diameter of the maximal inscribed circle efficiently. The maximal inscribed circle is a measure for the minimal primary particle size in one dimension and is shown to be commutable with Feret min measurements. The standard method to determine the fractal properties and the volume specific surface area of fractal-like aggregates is refined supporting on the diameter of the maximal inscribed circle and the overlap coefficient measurements for each individual primary particle. (C) 2014 Elsevier B.V. All rights reserved.