Increased sensitivity to concanavalin A and a suppressor cell defect in chronic active hepatitis.
Increased sensitivity to concanavalin A and a suppressor cell defect in chronic active hepatitis.
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慢性活动性肝炎对伴刀豆球蛋白 A 的敏感性增加和抑制细胞缺陷。
DOI：
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发表时间：
1981
影响因子：
4.6
通讯作者：
R. Williams
中科院分区：
文献类型：
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作者：
C. de Galocsy;P. Jenkins;G. Mieli;A. Eddleston;R. Williams
对11例HBs Ag阴性的慢性活动性肝炎患者在临床和组织学上处于非活动期和复发期间，采用短期抑制性T细胞检测方法，对其抑制性T细胞活性进行了连续研究。在正常受试者中刀豆蛋白A的增殖反应不佳的情况下，当有丝分裂原的加入在淋巴细胞培养开始后推迟24小时时，胸腺嘧啶核苷掺入的预期增加，这一发现与最初孵育期间功能抑制细胞的消失一致。这一效应在正常受试者和非活动性疾病患者中都可以看到，但在复发时没有观察到。进一步的研究表明，在这些时间段，淋巴细胞对刀豆蛋白A的敏感性显著增加，这一发现可能解释了抑制细胞活性明显下降的原因。然而，当使用较低浓度的丝裂原时，在慢性活动性肝炎患者中观察到抑制细胞活性的显著降低，在活动组和非活动组中几乎相同，这表明在这种疾病中存在真正的抑制细胞缺陷。
Suppressor T cell activity was studied serially in 11 patients with HBsAg-negative chronic active hepatitis when the disease was clinically and histologically inactive and during relapse using a short-lived suppressor cell assay. With a concentration of concanavalin A which gave a suboptimal proliferative response in normal subjects, the expected increase in thymidine incorporation was seen when the addition of the mitogen was delayed for 24 hr after the start of the lymphocyte culture period, a finding consistent with the disappearance of functional suppressor cells during the initial incubation. This effect was seen both in normal subjects and in patients with inactive disease, but was not observed at times of relapse. Further studies revealed a marked increase in lymphocyte sensitivity to concanavalin A at these times, a finding which might explain the apparent decrease in suppressor cell activity. When lower concentrations of mitogen were used in the assay, however, a significant reduction in suppressor cell activity was observed in the patients with chronic active hepatitis which was almost equal in magnitude in both the active and inactive groups, suggesting the presence of a true suppressor cell defect in this disease.