Alteration of fatty acid metabolism in the liver, adipose tissue, and testis of male mice conceived through assisted reproductive technologies: fatty acid metabolism in ART mice.
Alteration of fatty acid metabolism in the liver, adipose tissue, and testis of male mice conceived through assisted reproductive technologies: fatty acid metabolism in ART mice.
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通过辅助生殖技术受孕的雄性小鼠肝脏、脂肪组织和睾丸脂肪酸代谢的改变：ART小鼠的脂肪酸代谢
DOI：
10.1186/1476-511x-12-5
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发表时间：
2013-01-23
影响因子：
4.5
通讯作者：
Jin F
中科院分区：
文献类型：
--
作者：
Wang LY;Le F;Wang N;Li L;Liu XZ;Zheng YM;Lou HY;Xu XR;Chen YL;Zhu XM;Huang HF;Jin F
研究背景脂代谢在妊娠的整个过程中起着重要的作用。先前的研究已经证明了通过辅助生殖技术（ART）获得的妊娠胎盘中的脂质代谢异常。因此，我们推测ART显微操作可能影响子代的脂质代谢，并在本研究中重点关注ART雄性子代的脂肪酸代谢。比较自然受孕（NC）、控制性超排卵（COH）、体外受精（IVF）和卵胞浆内单精子注射（ICSI）小鼠，分析ART对子代的影响。本研究的小鼠模型分为两个年龄组：成年组和老年组。结果ART组小鼠肝脏和脂肪组织中脂肪酸组成发生明显变化，睾丸中脂肪酸组成无明显变化。在脂肪组织中，ART小鼠在成年和老年小鼠中均表现出单不饱和脂肪酸（MUFA）减少和多不饱和脂肪酸（PUFA）增加，而成年小鼠中饱和脂肪酸（SFA）的变化在老年小鼠中消失。在肝脏中，成年小鼠的变化更为复杂，而ART老年小鼠的MUFAs增加和PUFAs减少。ART组脂肪酸代谢相关酶活性及脂肪生成和脂肪分解蛋白表达均发生变化，成年小鼠和老年小鼠的变化不一致。进一步分析表明，脂肪酸与脂肪酸代谢相关酶活性的变化以及脂肪生成和脂肪分解蛋白的表达密切相关。此外，我们还发现，单独ART治疗对脂肪酸代谢的影响因年龄和组织而异。结论ART治疗对雄性小鼠脂肪组织和肝脏中的脂肪酸组成有影响。脂肪酸含量的变化是调节脂肪酸组成的关键。这些变化可能对ART雄性后代的健康产生潜在影响，需要进一步研究。
BackgroundLipid metabolism plays important roles in the whole process of pregnancy. Previous studies have demonstrated abnormalities of lipid metabolism in the placentas of pregnancies obtained by assisted reproductive technology (ART). Therefore, we hypothesized that ART micromanipulation may affect lipid metabolism in offspring, and focused on the fatty acid metabolism in ART male offspring in this study.MethodsThe fatty acid metabolism in the liver, adipose tissue and testis was detected. The comparison between naturally conceived (NC), controlled ovarian hyperstimulation (COH),in vitrofertilization (IVF) and intracytoplasmic sperm injection (ICSI) mice was made to analyze the effect of ART on offspring. The mice models in this study included two age groups: adult group and old group. The fatty acid composition and the expression of lipid metabolism-related genes were analyzed by GC-MS and qRT-PCR.ResultsThe fatty acid composition in the liver and adipose tissue were significantly altered in ART mice, but no significant difference was found in the testis. In adipose tissue, ART mice showed decreased monounsaturated fatty acids (MUFAs) and increased polyunsaturated fatty acids (PUFAs) in both adult and old mice, while the alteration of saturated fatty acids (SFAs) in the adult disappeared in the old. In liver, the changes were much complex in adult mice, while increased MUFAs and decreased PUFAs were found in ART old mice. The activities of fatty acid metabolism-related enzymes and the expression of lipogenic and lipolytic proteins changed in ART groups, with the adult mice and old mice showing inconsistent alterations. Further analysis indicated that SFAs was closely associated with the alterations of fatty acid metabolism-related enzyme activities and the expression of lipogenic and lipolytic proteins. Furthermore, we also found that the effect of separated ART treatments on fatty acid metabolism varied with different ages and tissues.ConclusionsART treatments had effect on the fatty acid composition in adipose tissue and liver of male mice. The alteration of SFAs content was crucial for the regulation of fatty acid composition. These changes might have potential effects on the health of ART male offspring which need further investigation.