The ETT2 transcriptional regulator EivF affects the serum resistance and pathogenicity of avian pathogenic Escherichia coli
The ETT2 transcriptional regulator EivF affects the serum resistance and pathogenicity of avian pathogenic Escherichia coli
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ETT2转录调节因子EivF影响禽致病性大肠杆菌的血清耐药性和致病性。
DOI：
10.1016/j.micpath.2021.105261
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发表时间：
2021-11-01
影响因子：
3.8
通讯作者：
Qi, Kezong
中科院分区：
文献类型：
--
作者：
Fu, Dandan;Li, Jiaxuan;Qi, Kezong
禽致病性大肠埃希氏菌(APEC)是肠道外致病性大肠杆菌(ExPEC)的一种病原型，可引起严重的禽类全身性传染病。大肠埃希菌III型分泌系统2(ETT2)广泛存在于包括ExPEC和EHEC在内的大肠杆菌菌株中。位于ETT2簇的转录调控因子EivF在EHEC O157：H7中影响Lee编码蛋白的分泌，并增加细菌对肠上皮细胞的黏附。在之前的研究中，我们证明了转录调控因子可以影响APEC的运动性和生物膜的形成。在这里，我们评估了EivF是否参与了APEC的致病过程，我们发现EIVF的灭活显著增强了对血清的抵抗力、对鸡胚成纤维细胞(DF-1)的黏附，以及APEC在雏鸡体内的定植能力。为了进一步阐明转录调控因子EivF的调控机制，我们对差异表达的基因和途径进行了分析，结果表明EivF调控膜、黏附、环境胁迫和分泌蛋白基因，并参与定位、生物黏附、生物调节、膜和毒素活性。这些发现表明，ETT2转录调控因子EivF作为负抑制因子在APEC的致病过程中起着关键作用。
Avian pathogenic Escherichia coli (APEC), a pathotype of extraintestinal pathogenic Escherichia coli (ExPEC), can cause serious systemic infectious diseases in poultry. Escherichia coli type III secretion system 2 (ETT2) is widely distributed in E. coli strains, including ExPEC and Enterohemorrhagic Escherichia coli (EHEC). The transcriptional regulator EivF, which is located at the ETT2 cluster, affects the secretion of LEE-encoded proteins and increases bacterial adhesion to human intestinal epithelial cells in EHEC O157:H7. In a previous study, we demonstrated the transcriptional regulator can affect APEC's motility and biofilm formation. Here, we evaluated whether EivF is involved in the pathogenicity of APEC, and we found that inactivation of eivF significantly enhanced resistance to the serum, adherence to chicken embryo fibroblast (DF-1) cells, and the colonization ability of APEC in chicks. To further clarify the regulation mechanism of transcriptional regulator EivF, we performed transcriptome sequencing to analyze the differentially expressed genes and pathways, showing that EivF regulates membrane, adhesion, environmental stress, and secretion protein genes, and EivF is involved in the localization, biological adhesion, biological regulation, membrane, and toxin activity. These findings indicated that the ETT2 transcriptional regulator EivF plays a crucial role in the pathogenicity of APEC as a negative repressor.