TRANSCRIPTION OF HUMAN PAPILLOMAVIRUS TYPE-16 EARLY GENES IN A CERVICAL-CANCER AND A CANCER-DERIVED CELL-LINE AND IDENTIFICATION OF THE E7-PROTEIN
TRANSCRIPTION OF HUMAN PAPILLOMAVIRUS TYPE-16 EARLY GENES IN A CERVICAL-CANCER AND A CANCER-DERIVED CELL-LINE AND IDENTIFICATION OF THE E7-PROTEIN
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DOI：
10.1073/pnas.83.13.4680
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发表时间：
1986-07-01
影响因子：
11.1
通讯作者：
WETTSTEIN, FO
中科院分区：
文献类型：
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作者：
SMOTKIN, D;WETTSTEIN, FO
人乳头瘤病毒16型DNA和RNA的特点是在宫颈癌衍生的CaSki细胞系，其中只含有整合的DNA，并在宫颈癌，其中主要含有质粒DNA。在这两种情况下，主要RNA可以编码早期开放阅读框架E7，次要RNA可以编码E6。宫颈癌，而不是CaSki细胞系，含有一个小的RNA，可以编码一个完整的E2蛋白，这可能与质粒DNA的持续存在。RNA图谱数据表明，poly（A）+ RNA是从存在的几百个基因拷贝中的一小部分转录的，并且在宫颈癌中，这些基因组似乎是整合的。E7蛋白已在CaSki细胞中被鉴定，其mRNA的流行表明其可能在恶性状态的进展或长期维持中起作用。
Human papillomavirus type 16 DNA and RNA were characterized in the cervical cancer-derived CaSki cell line, which contains only integrated DNA, and in a cervical cancer, which contains predominantly plasmid DNA. In both, a major RNA can code for the early open reading frame E7 and a minor one can code for E6. The cervical cancer, but not the CaSki cell line, contains a minor RNA that can code for an intact E2 protein, and this may relate to the continued presence of plasmid DNA. The RNA mapping data suggest that the poly(A)+ RNA is transcribed from a minor fraction of the several hundred gene copies present, and in the cervical cancer these genomes appear to be integrated. The E7 protein has been identified in CaSki cells and the prevalence of its mRNA suggests a possible function in progression to, or long-term maintenance of, the malignant state.