Chlamydia trachomatis tarp is phosphorylated by src family tyrosine kinases
Chlamydia trachomatis tarp is phosphorylated by src family tyrosine kinases
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DOI：
10.1016/j.bbrc.2008.04.089
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发表时间：
2008-06-27
影响因子：
3.1
通讯作者：
Hackstadt, Ted
中科院分区：
文献类型：
--
作者：
Jewett, Travis J.;Dooley, Cheryl A.;Hackstadt, Ted
在沙眼衣原体附着于靶细胞后不久，将易位的肌动蛋白募集磷蛋白（Tarp）注入细胞质中，随后被一种未鉴定的酪氨酸激酶磷酸化。Tarp磷酸化在细菌进入中的作用尚不清楚。在这项研究中，重组沙眼衣原体Tarp用于鉴定磷酸化所需的宿主细胞激酶。L2 Tarp的每个富含酪氨酸的重复序列含有与Src和AN激酶共有靶相似的序列。此外，纯化的p60-src、Yes、Fyn和Abl激酶能够磷酸化Tarp。单个富含酪氨酸的重复肽中潜在酪氨酸的突变表明Src和Abl激酶磷酸化相同的残基，这表明C.沙眼衣原体Tarp可作为多种宿主细胞激酶的底物。令人惊讶的是，Src和Abl激酶的化学抑制阻止了培养物中Tarp的磷酸化，并且对细菌进入宿主细胞没有可测量的影响。爱思唯尔公司出版
The translocated actin recruiting phosphoprotein (Tarp) is injected into the cytosol shortly after Chlamydia trachomatis attachment to a target cell and subsequently phosphorylated by an unidentified tyrosine kinase. A role for Tarp phosphorylation in bacterial entry is unknown. In this study, recombinant C trachomatis Tarp was employed to identify the host cell kinase(s) required for phosphorylation. Each tyrosine rich repeat of L2 Tarp harbors a sequence similar to a Src and AN kinase consensus target. Furthermore, purified p60-src, Yes, Fyn, and Abl kinases were able to phosphorylate Tarp. Mutagenesis of potential tyrosines within a single tyrosine rich repeat peptide indicated that both Src and Abl kinases phosphorylate the same residues suggesting that C. trachomatis Tarp may serve as a substrate for multiple host cell kinases. Surprisingly, chemical inhibition of Src and Abl kinases prevented Tarp phosphorylation in culture and had no measurable effect on bacterial entry into host cells. Published by Elsevier Inc.