Co-operation of interleukin-1 beta and tumour necrosis factor-alpha in the activation of human umbilical vein endothelial cells to inhibit Toxoplasma gondii replication.
Co-operation of interleukin-1 beta and tumour necrosis factor-alpha in the activation of human umbilical vein endothelial cells to inhibit Toxoplasma gondii replication.
复制标题
白细胞介素-1β和肿瘤坏死因子-α协同激活人脐静脉内皮细胞抑制弓形虫复制。
作者：
I. Dimier;D. Bout
The tachyzoites of the RH strain of Toxoplasma gondii invaded and replicated in cultured human umbilical vein endothelial cells (HUVEC). Co-treatment of the HUVEC with human recombinant interleukin-1 beta (IL-1 beta) and tumour necrosis factor-alpha (TNF-alpha) inhibited the replication of T. gondii. Growth of the parasite was measured in vitro by [3H]uracil incorporation assay 18 hr after infection. This assay showed that when cells were pretreated with IL-1 beta and TNF-alpha concentrations ranging from 1 to 100 U/ml, a high degree of inhibition was observed with a phenomenon of co-operation causing a dose-dependent inhibition of the replication of intracellular T. gondii. The mechanism(s) by which the inhibition of multiplication was effected remains to be elucidated.
