In vitro propagation of Tacca chantrieri
In vitro propagation of Tacca chantrieri
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发表时间：
2002
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通讯作者：
Z. Yanjun
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作者：
Z. Yanjun
由于长叶薯种子多，但能萌发的种子很少（约12%），因此组织培养是长叶薯繁殖的一种有效途径。本试验以山慈菇成熟种子的幼叶和叶柄为外植体进行离体繁殖，其适宜的培养条件为：种子萌发培养基为MS+6 BA 1 mg/L+ NAA 0 1 mg/L，愈伤组织诱导培养基为MS +6 BA 1 mg/L+2，4 D 1 5 mg/L+ KT 0 2 mg/L，不定芽诱导的培养基为MS+6 BA 0 5 mg/L+NAA 0 5 mg/L，生根培养基为1/2 MS+NAA 0 5 mg/L。
Tacca chantrieri bear many seeds,but only few of the seeds (about 12%) can germinate,so tissue culture would be a good means for multiplying plants of T.chantrieri. In this experiment,young leaves and leaf stalks,obtained from mature T.chantrieri seeds,were used as explants for in vitro propagation.The appropriate media for respective culture stages were as follows.The medium of seed germinarion was MS+6 BA 1mg/L+NAA0 1mg/L,that of callus inducing was MS+6BA1mg/L+2,4D1 5mg/L+KT0 2mg/L,that of shoot inducing was MS+ 6BA 0 5mg/L+NAA 0 5 mg/L,that of rooting was 1/2 MS+NAA 0 5 mg/L.