The effect of prenatal nicotine on mRNA of central cholinergic markers and hematological parameters in rat fetuses
The effect of prenatal nicotine on mRNA of central cholinergic markers and hematological parameters in rat fetuses
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DOI：
10.1016/j.ijdevneu.2008.02.007
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发表时间：
2008-08-01
影响因子：
1.8
通讯作者：
Xu, Zhice
中科院分区：
文献类型：
--
作者：
Mao, Caiping;Yuan, Xin;Xu, Zhice
许多研究已经证明尼古丁对胎儿发育的影响。本研究测定了大鼠胎仔在慢性产前尼古丁暴露后前脑和后脑中胆碱乙酰转移酶（ChAT）、囊泡乙酰胆碱转运体（VAChT）和高亲和力胆碱转运体（CHT 1）的表达（在不同妊娠期皮下注射尼古丁）。我们还测量了长期尼古丁暴露对胎儿血液pO（2）、pCO（2）、pH、Na+和K+浓度以及乳酸水平的影响。孕妇在怀孕期间接触尼古丁与胎儿pO（2）降低、pCO（2）和乳酸显著增加以及胎儿生长受限有关。此外，母体尼古丁给药还降低了胎儿脑中ChAT、VAChT和CHT 1 mRNA水平。尼古丁诱导的胎儿缺氧反应和减少胆碱能标记物的表达在大脑中更严重时，尼古丁开始在妊娠早期。我们的研究结果提供了新的信息重复暴露于尼古丁在子宫内的影响，中央ChAT，VAChT，和CHT 1在大鼠胎儿的表达。这些结果表明，反复缺氧发作或/和尼古丁对中枢胆碱能系统的直接影响，在怀孕期间可能会导致胎儿起源的脑发育问题。(C)2008 ISDN。由爱思唯尔有限公司发布。保留所有权利。
A number of studies have demonstrated the influence of nicotine on fetal development. This study determined the expression of choline acetyltransferase (ChAT), vesicular acetylcholine transporter (VAChT), and high-affinity choline transporter (CHT1) in the forebrain and hindbrain following chronic prenatal nicotine exposure in the rat fetus (maternal rats were subcutaneously injected with nicotine at different gestation periods). We also measured the effect of chronic nicotine exposure on fetal blood pO(2), pCO(2), pH, Na+ and K+ concentrations, as well as lactic acid levels. Maternal nicotine exposure during pregnancy was associated with a decrease in fetal pO(2) coupled with a significant increase in pCO(2) and lactic acid as well as restricted fetal growth. Additionally, maternal nicotine administration also reduced ChAT, VAChT, and CHT1 mRNA levels in the fetal brain. Nicotine-induced fetal hypoxic responses and reduced cholinergic marker expression in the brain were more severe when nicotine was started in early gestation. Our results provide new information about the effects of repeated exposure to nicotine in utero on the expression of central ChAT, VAChT, and CHT1 in the rat fetus. These results indicate that repeated hypoxic episodes or/and a direct effect of nicotine on the central cholinergic system during pregnancy may contribute to brain developmental problems in fetal origin. (C) 2008 ISDN. Published by Elsevier Ltd. All rights reserved.