Postnatal development of electrical and enzyme histochemical activity in Purkinje cells.
Postnatal development of electrical and enzyme histochemical activity in Purkinje cells.
复制标题
浦肯野细胞的电和酶组织化学活性的出生后发育。
DOI：
10.1016/0014-4886(69)90039-9
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发表时间：
1969
影响因子：
5.3
通讯作者：
Lowell W. Lapham
中科院分区：
文献类型：
--
作者：
Donald J. Woodward;Barry J. Hoffer;Lowell W. Lapham
记录了出生后数小时至成年的大鼠浦肯野细胞的胞外动作电位，分别用Nembutal麻醉和Curare麻痹。组织化学方法测定乳酸、琥珀酸脱氢酶、γ-氨基丁酸-α-酮戊二酸转氨酶活性。动作电位在出生后几小时即可观察到，1-2天开始有规律的持续自发活动。所有三种酶的活性在0-3天之间急剧增加，仅限于小脑皮质的浦肯野细胞胞体。这些酶的活性在7日龄达到最大值。7天后观察到自发的爬行纤维爆发。在出生后10天到15天之间，自发放电频率增加，尖峰持续时间缩短，放电模式从规则转变为不规则。此时，在分子层神经束、肾小球和小脑中间神经元中同时出现组织化学活性。
Extracellular action potentials were recorded from Purkinje cells of rats, from several hours after birth to adult in age, anesthetized with Nembutal and paralyzed with curare. Lactate and succinate dehydrogenase and γ-amino butyrate-α-ketoglutarate transaminase activities were histochemically determined using tetrazolium reduction techniques. Action potentials were observed as early as several hours after birth with regular sustained spontaneous activity beginning at 1–2 days. There is a sharp increase in activity of all three enzymes between 0 and 3 days restricted in the cerebellar cortex to Purkinje-cell soma. Maximum activities of these enzymes were found at 7 days. Spontaneous climbing-fiber bursts were observed by 7 days. Between 10 and 15 days after birth there occurs an increase in spontaneous firing rates, a decrease in spike duration, and a shift from regular to irregular discharge patterns. There is a simultaneous appearance of histochemical activity in the neuropil of the molecular layer, the glomeruli, and the cerebellar interneurons at this time.