Isolated mouse islets as a model for studying insulin release
Isolated mouse islets as a model for studying insulin release
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分离的小鼠胰岛作为研究胰岛素释放的模型
DOI：
10.1007/bf01550894
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Å. Lernmark
中科院分区：
文献类型：
--
作者：
Å. Lernmark
用显微分离的小鼠胰岛体外系统研究胰岛素的释放。考虑到其高含量的功能充分的β-细胞，认为来自肥胖-高血糖小鼠的胰岛特别有用。在冷冻干燥和称重每个孵育的胰岛后，可以表示每胰岛干重的胰岛素释放速率。从单个孵育的胰岛释放的胰岛素可以使用结晶小鼠胰岛素作为标准进行放射免疫测量。研究了各种因素的影响，包括释放的胰岛素的扩散、胰岛大小、摇动、温度、pH、渗透强度、氧张力和酶处理。如果基础率保持较低，则可以获得葡萄糖刺激的胰岛素释放的早期和晚期的可重复值。暴露于胶原酶的微切胰岛倾向于增加胰岛素释放的基础速率，但并没有显着影响总胰岛素对葡萄糖的反应，也没有随之而来的上升，在胰岛水平的果糖-1，6-二磷酸。该体外系统为药物筛选提供了一个有用的模型，用于各种化合物的胰岛素释放能力。同样重要的是，从单个胰岛释放胰岛素的速率可以与其代谢直接相关。
Anin vitrosystem with microdissected mouse islets was employed for studying insulin release. Islets from obese-hyperglycemic mice were considered particularly useful in view of their high content of adequately functioning β-cells. After freeze-drying and weighing each of the incubated islets it was possible to express the rate of insulin release per islet dry weight. Insulin released from a single incubated islet could be measured radioimmunologically using crystalline mouse insulin as standard. The effects of various factors were examined, including diffusion of released insulin, islet size, shaking, temperature, pH, osmotic strength, oxygen tension, and treatment with enzymes. Provided that the basal rate was kept low, reproducible values could be obtained for both the early and the late phases of the glucose-stimulated insulin release. Exposure of the microdissected islets to collagenase tended to increase the basal rate of insulin release but did not significantly affect the total insulin response to glucose nor the concomitant rise in the islet level of fructose-1,6-diphosphate. The presentin vitrosystem offers a useful model for pharmacological screening on the insulin-releasing capacity of various compounds. It is also important that the rate of insulin release from the individual islet can be directly related to its metabolism.