The marine bacterium Pseudoalteromonas haloplanktis has a complex genome structure composed of two separate genetic units.
The marine bacterium Pseudoalteromonas haloplanktis has a complex genome structure composed of two separate genetic units.
复制标题
海洋细菌盐生假交替单胞菌具有复杂的基因组结构，由两个独立的遗传单位组成。
DOI：
10.1101/gr.6.12.1160
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发表时间：
1996
期刊：
影响因子：
7
通讯作者：
Giovannoni,SJ
中科院分区：
文献类型：
--
作者：
Lanoil,BD;Ciuffetti,LM;Giovannoni,SJ
盐浮游假互单胞菌是一种普遍存在且易于培养的海洋细菌，它的基因组大小被测量为估计海洋浮游细菌基因组复杂性的一步。为了确定总基因组的大小，我们用限制性内切酶Notl和Sfil消化了盐浮游藻DNA，用脉冲场凝胶电泳（PFGE）分离了片段，并对片段的大小进行了总结。盐浮游藻基因组经Notl酶切为3512 +/- 112 kb，经Sfil酶切为3468 +/- 54.1 kb。浮游藻也被证明具有复杂的基因组结构，由两个大约2700和800 kb的大复制子组成。有三个证据支持这一结论：(1)在未消化的盐浮游藻DNA的PFGE中，总是可以看到两个独立的条带；(2)与全基因组DNA的酶切酶切相比，较大条带的酶切酶切缺失约650 kb；(3) 16S rDNA探针与较大的复制子杂交而不与较小的复制子杂交。据我们所知，浮游藻是第一个具有复杂基因组结构的海洋细菌。
The genome size of Pseudoalteromonas haloplanktis, a ubiquitous and easily cultured marine bacterium, was measured as a step toward estimating the genome complexity of marine bacterioplankton. To determine total genome size, we digested P. haloplanktis DNA with the restriction endonucleases Notl and Sfil, separated the fragments using pulsed-field gel electrophoresis (PFGE), and summed the sizes of the fragments. The P. haloplanktis genome was 3512 +/- 112 kb by Notl digestion and 3468 +/- 54.1 kb by Sfil digestion. P. haloplanktis is also shown to have a complex genome structure, composed of two large replicons of approximately 2700 and 800 kb. Three pieces of evidence support this conclusion: (1) Two separate bands are always seen in PFGE of undigested P. haloplanktis DNA; (2) restriction digests of the larger band are missing a band of approximately 650 kb compared with restriction digests of total genomic DNA; and (3) a 16S rDNA probe hybridized to the larger replicon but not to the smaller. To our knowledge, P. haloplanktis is the first marine bacterium shown to have a complex genome structure.